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Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

17.8K Views

10:25 min

March 8th, 2017

March 8th, 2017

17,840 Views

0:05

Title

1:01

Skeletal Muscle Homogenization and Mitochondrial Isolation

4:00

High-resolution Respirometry

4:44

Complex I-dependent Respiration, Complex II-dependent Respiration, and Complex IV-dependent Respiration

6:51

Results: High-resolution Respirometry Measurements from Intact Mitochondria Isolated from Skeletal Muscle

8:35

Conclusion

Transcrição

The overall goal of this procedure is to isolate skeletal muscle mitochondria by differential centrifugation and determine the respiratory rates of mitochondrial respiratory chain complexes I, II and IV.This method can help answer key questions in

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Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.

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