Results: Protein Inclusions Play a Protective Role Against Oxidative Stress
10:38
Conclusion
TRANSCRIÇÃO
The overall goal of this procedure is to determine the relationship between the formation of intracellular protein aggregates and their impact on cellular oxidative stress using the baker's yeast Saccharomyces cerevisiae. This method can help answ
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Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.