Cloning and Purification of Untagged Human TET2 Dioxygenase
5:30
5mC Oxidation by TET2 Dioxygenase
7:02
Quantitative Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-based Assay Development
8:31
Results: Investigating the Role of the TET2 Enzyme in Normal Development and Disease
9:26
Conclusion
TRANSCRIÇÃO
This method can help answer key questions in epigenetics and TET2 mediated 5-Methylcytosine demethylation field such an analysis of activity of normal and clinical TET2 mutations. The main advantage of this technique is that native TET2 can be pur
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Here, we present a protocol for an efficient single step purification of the active untagged human ten-eleven translocation-2 (TET2) 5-methylcytosine dioxygenase using ion-exchange chromatography and its assay using a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based approach.