Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

0:46

Expression of His₆-TIp3-LBD in E. coli

2:29

Isolation and Denaturation of Inclusion Bodies

5:34

Protein Refolding

7:39

Purification of His₆-tagged Protein Using Immobilized Metal Ion Affinity Chromatogrphy

8:49

His₆-tag Removal Using TEV Protease and Size-excluson Chromatography of Tlp3-LBD

11:06

Results: Expression, Refolding, and Purification of Tlp3-LBD

12:41

Conclusion