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JoVE Journal

Immunology and Infection

This content is Open Access.

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

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09:26 min

June 14th, 2018

June 14th, 2018

8,510 Views

0:04

Title

0:51

Preparing the Pupae

3:12

Laser-induced Wounding of Drosophila Pupal Wings

4:41

In Vivo Time-lapse Confocal Imaging

6:52

Results: Sterile Wounding Activates a Robust Inflammatory Response in the Drosophila Pupal Wing

8:28

Conclusion

Transcrição

Here we present a protocol for live-imaging wound repair and the associated inflammatory response at high spatio-temporal resolution in vivo. This method utilizes the pupal stage of Drosophila development to enable long-term imaging and tracking of specific cell populations over time and is compatible with efficient RNAi-mediated gene inactivation.

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