Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
2:24
Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
7:02
Results: Analysis of Plant Proteins Secreted by Insect Cells
7:57
Conclusion
TRANSCRIÇÃO
This method can help answer key questions in the biochemistry, and structural biology field of secreted proteins, such as protein structure determination of, by crystallography and cryo-EM. The main advantage of this technique is it produces large
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Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.