Seed hPSCs onto Plates at Defined Densities for Differentiation
4:39
Differentiation of hPSCs into Endodermal Cells and Liver Progenitors
5:37
Results: Differentiating Human Pluripotent Stem Cells into Liver Cells
6:58
Conclusion
Transcrição
This method enables the generation of large numbers of human liver bud progenitors and hepatocyte-like cells with high purity. The ability to generate these liver cells could be important for regenerative medicine and other fields. By controlling
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This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.