The authors would like to acknowledge support from the EPSRC via X. Zhao&#39;s reactive inkjet printing of silk materials awards (EP/N007174/1 and EP/N023579/1), S. J. Ebbens Career Acceleration Fellowship, and Healthcare Technology Impact Fellowship (EP/J002402/1 and EP/N033736/1). The authors also thank Dr. Qingyou Xia from the State Key Laboratory of Silkworm Genome Biology, Southwest University, China for providing silk cocoons.

CAUTION: Please consult relevant material safety datasheets before use of hydrogen peroxide, sodium carbonate, ethanol, calcium chloride, and methanol. Ensure to wear all appropriate personal protective equipment including engineering controls while handling chemicals used in this protocol.
1. Extraction of fibroin
<ol>
	<li>Cut 5 g of cleaned silk cocoons into ~ 1 cm2 small pieces using scissors.</li>
	<li>Boil 2 L of deionized (DI) water in a 2 L beaker on a magnetic hot plate under an extraction hood.</li>
	<li>Add 4.24 g of sodium carbonate gradually and slowly into the boiling water to avoid boiling over and let it dissolve with the help of a magnetic stir bar.</li>
	<li>Wait until the solution starts to boil again and add the cut pieces of cocoons into the solution. Ensure that all the silk is submerged in the solution and keep the solution boiling under constant stirring for 90 min. Cover the beaker lightly with aluminium foil and top up with preheated DI water regularly to replenish water loss due to evaporation.</li>
</ol>
2. Drying of fibroin
<ol>
	<li>Remove the extracted fibroin fibers from the sodium carbonate solution with a glass rod or spatula and wash 3x with 1 L of preheated DI water for each wash, gradually decreasing the temperature for every washing step (approximately 60 &#176;C, 40 &#176;C, and room temperature, 25 &#176;C).</li>
	<li>Spread out the fibroin fibers on a 750 mL borosilicate glass crystallizing dish and place it into a drying oven at 60 &#176;C under atmospheric pressure and leave to dry overnight. Once dried, store the fibroin in a closed container at room temperature.</li>
</ol>
3. Dissolution of fibroin
<ol>
	<li>Prepare a ternary solution (Ajisawa&#8217;s reagent) containing 4.8 g of DI water, 3.7 g of ethanol, and 3.1 g of calcium chloride17.</li>
	<li>Place a two-neck round bottom flask (100 mL) into a water bath, made by filling a 750 mL borosilicate glass crystallizing dish with 600 mL of DI water, on top of a magnetic hot plate. Place the ternary solution inside the flask.</li>
	<li>Place a thermometer in one of the necks to monitor the solution temperature accurately. Cover the other neck with aluminium foil to prevent drying out of the solution due to evaporation (or use a water-cooled reflux condenser). Heat up the solution to 80 &#176;C. 
	NOTE: Ensure that the bulb of the thermometer is inside the solution.</li>
	<li>When the temperature of the solution is stable at 80 &#176;C, remove the aluminium foil and add 1 g of dried fibroin to the solution. Add a small magnetic stir bar to ensure that the solution is mixed well throughout the dissolution process. Cover the second neck again with aluminium foil to minimize evaporation but keep the system open. Leave to dissolve for 90 min.</li>
</ol>
4. Dialysis of fibroin solution
<ol>
	<li>After 90 min of dissolution, leave the fibroin solution for 10 min to cool down to room temperature.</li>
	<li>Take one 15 cm long dialysis tube (molecular weight cut-off 12,000&#8722;14,000 kDa) and tie a knot in one of the two ends. Wash it for a few minutes with running DI water from the tap.</li>
	<li>Open the other end and pour the fibroin solution inside. Using a metal clamp, close the other end of the dialysis tube ensuring that the tube is closed as tightly as possible. Attach one of the ends of the dialysis tube via a screw cap to an empty 30 mL plastic vial to allow the dialysis tube to float in the water.</li>
	<li>Fill a 2 L beaker with 2 L of DI water and place the dialysis tube inside it. Change the water at regular intervals. Check the conductivity of the water every time it is changed to follow the dialysis process. The dialysis step finishes once the conductivity of the water is below 10 &#181;S/cm. 
	NOTE: This process usually takes around 24&#8722;36 h with 5 changes of water.</li>
	<li>After dialysis is complete, cut one end of the dialysis tube with scissors and pour the solution into a series of 1.5 mL tubes. Then, centrifuge for 5 min at 16,000 x g to remove any particles inside the fibroin solution. Collect the supernatant in a 30 mL plastic vial and store it at 4 &#176;C.</li>
</ol>
5. Determination of RSF solution concentration
<ol>
	<li>Weigh a clean glass slide (W1). Add 200 &#181;L of silk solution (V1).</li>
	<li>Leave the glass slide in an oven at 60 &#176;C for 2 h.</li>
	<li>Weigh the glass slide again (W2).</li>
	<li>Calculate the concentration of the silk solution (w/v) using the following formula: 
	<img alt="Equation 1" src="/files/ftp_upload/59030/59030eq1.jpg" /></li>
</ol>
6. Preparation of inks for printing
<ol>
	<li>Prepare ink A (final volume 1.5 mL) by mixing the fibroin solution (40 mg/mL), polyethylene glycol 400 (PEG400; 14 mg/mL) and deionized water for printing the main body of the SPMSs.</li>
	<li>For printing the catalytic engine of the SPMSs, mix fibroin (40 mg/mL), PEG400 (12 mg/mL),&#160;catalase (6 mg/mL with catalytic activity of &#62;20,000 units/mg) and deionized water to make 1.5 mL of ink B.</li>
	<li>Prepare 1.5 mL of ink C by dissolving Coomassie brilliant blue (0.05 mg/mL) in methanol. 
	NOTE: Methanol is used for converting the fibroin random coils to rigid beta sheets by printing ink C on top of ink A or ink B. Coomassie brilliant blue is used to provide a contrasting color of SPMSs to aid auto tracking of the SPMSs during propulsion.</li>
</ol>
7. Reactive inkjet 3D printing
NOTE: The inkjet printer used in these experiments is based on piezo actuated jetting devices with glass nozzles. There are several commercially available inkjet printers for research that can duplicate these functions.
<ol>
	<li>Use jetting devices with 80 &#956;m nozzle diameter for printing the inks on a silicon substrate placed on the stage at a working distance between the nozzle and the Si-wafer substrate of around 5 mm. The geometric shapes of the SPMSs are digitally defined as a series of points of X-Y coordinates in a spreadsheet file. 
	NOTE: The printer reads the coordinates serially and runs the printer accordingly. Each coordinate point makes the printer jet once through the jetting device. Separate spreadsheet files are created for inks A and B (see Supplemental Files [SPMS Main Body.xlsx and SPMS Engine.xlsx]).</li>
	<li>Load the three inks (A, B, and C) into three reservoirs (1.5 mL each) and then adjust the backpressure using the backpressure valve for each individual channel to ensure that the ink is not dripping from the jetting devices. 
	NOTE: Three jetting devices on independent channels are needed.</li>
	<li>Adjust the jetting parameters (rise time 1, dwell time, fall time, eco time, rise time 2, idle voltage, dwell voltage, echo voltage) for each channel to ensure that each ink gives a good stable droplet formation (Figure 2). 
	NOTE: These parameters are jetting device and ink dependant and will need to be adjusted accordingly.</li>
	<li>Print the silk fibroin ink layer-by-layer alternating with methanol on clean polished Si-wafer substrates: stage 1, printing of ink A (main body); stage 2: printing of ink C (curing ink); stage 3: printing of ink B (catalytic ink for engine sites); stage 4: printing of ink C (curing ink); stage 5: repeat stages 1-4 for desired layers required (e.g., 100). 
	NOTE: Two example designs for the 4 stages are included in Supplemental Files; SPMS Main Body.xlsx is used for stage 1 and stage 2, and SPMS Engine.xlsx is used for stage 3 and stage 4.</li>
	<li>Print two batches of fibroin SPMSs with 200 layers and 100 layers thickness, respectively. 
	NOTE: The catalase engine is located on the side of one end of each stirrer. Thus, the stirrers have one catalytic engine (see Figure 1 red region).</li>
	<li>To remove the samples off the Si-wafers, immerse samples in DI water and gently agitate until detachment occurs.</li>
</ol>
8. Data acquisition/tracking and trajectory analysis of self-propelled stirrers
<ol>
	<li>Clean a glass petri dish (9 cm in diameter) with DI water ensuring that the surface is dust free.</li>
	<li>Once clean and dry, add 10 mL of pre-filtered (0.45 &#956;m) 5% w/v H2O2 into the petri dish and leave to settle. Light up the bottom of the petri dish with a cool white light-emitting diode (LED) light source and use a high-speed camera with macro zoom lens to capture the motion from above. Save videos as .avi files. 
	NOTE: See Table of Materials for details of the equipment used.</li>
	<li>Wash the printed silk stirrers for 10 min by submerging them in DI water to remove any unbound PEG400. Carefully take one washed stirrer with the tip of a sterile syringe needle and place it in the center of the petri dish. When the washed stirrer touches the H2O2 fuel, bubbles start forming around the engine and circular motion of the stirrer is observed. When the system appears stable (usually 10&#8722;30 s later), press record in the recording software to start capturing the video.</li>
	<li>Perform tracking of the micro stirrers on a frame by frame basis, tracking each end of the stirrers as indicated by points A and B in Figure 3. 
	NOTE: This can be done manually or with the aid of tracking software.</li>
	<li>From the obtained tracking data, calculate the instantaneous velocity between two consecutive frames (e.g., 1 and 2) using the equation below and average the resulting velocities from the entire sequence to obtain the mean instantaneous velocity.</li>
	<li>Further to this, calculate the angle of orientation &#966;. Then use the rate of change of &#966; to determine the rotational velocity (Figure 3). 
	<img alt="Equation 2" src="/files/ftp_upload/59030/59030eq2v3.jpg" /> 
	NOTE: When calculating instantaneous velocities from tracked image data, it is important that the initial image of an object with known dimensions is taken to be able to calculate correct pixel to micrometer values. These values will depend on the camera, objective, and distance used. Depending on the type of printed particle, choose different tracking points for calculating the velocity. For example, here tracking points A, B, and C (center of mass) are all used to determine instantaneous velocities (Figure 3).</li>
</ol>
9. Characterization of SPMSs by SEM
<ol>
	<li>Remove unused and used SPMSs from the Si-wafer or bulk solution and transfer them onto 10 mm wide carbon sticky pads mounted onto aluminium scan electron microscopy (SEM) stubs. Dry the samples in a drying oven for 10 min at 60 &#176;C.</li>
	<li>Load the sample stubs onto the sputter coater stage. Sputter coat (argon plasma at 0.05 Torr) 50&#8211;100 nm of gold onto the samples, ensuring a homogeneous gold surface coverage of the sample.</li>
	<li>Remove the sample stubs from the sputter coater and image in an SEM under vacuum at 5.0 kV. 
	NOTE: Very high acceleration voltages can burn the silk and give rise to false features.</li>
</ol>

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	<li>Gregory, D. A., Campbell, A. I., Ebbens, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+Catalyst+Distribution+on+Spherical+Bubble+Swimmer+Trajectories.">Effect of Catalyst Distribution on Spherical Bubble Swimmer Trajectories.</a> The Journal of Physical Chemistry C. 119 (27), 15339-15348 (2015).</li><li>Paxton, W. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Catalytic+Nanomotors:+Autonomous+Movement+of+Striped+Nanorods.">Catalytic Nanomotors: Autonomous Movement of Striped Nanorods.</a> Journal of the American Chemical Society. 126 (41), 13424-13431 (2004).</li><li>Gao, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Seawater-driven+magnesium+based+Janus+micromotors+for+environmental+remediation.">Seawater-driven magnesium based Janus micromotors for environmental remediation.</a> Nanoscale. 5 (11), 4696-4700 (2013).</li><li>Li, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Water-Driven+Micromotors+for+Rapid+Photocatalytic+Degradation+of+Biological+and+Chemical+Warfare+Agents.">Water-Driven Micromotors for Rapid Photocatalytic Degradation of Biological and Chemical Warfare Agents.</a> ACS Nano. 8 (11), 11118-11125 (2014).</li><li>Gregory, D. 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The authors have nothing to disclose.

A key feature of SPMSs produced in this protocol is the ability to quickly and easily design different shapes and structures via RIJ of silk doped with enzymes such as catalase and achieve chemically driven motion via bubble propulsion5. This together with the high biocompatibility18 of these stirrers makes them highly desirable for future applications for both water remediation challenges as well as for lab-on-a-chip applications for medical diagnosis devices.
Here, the propulsion ability is demonstrated by utilizing a simple line design with an engine part printed to the side of it as shown in Figure 1. The red dots represent the catalytically active catalase doped engine parts and the blue dots represent the inactive parts. To be able to generate 3D shapes via RIJ, it is necessary to print multiple layers in order to generate a z-axes height. Here, the numbers of layers deposited onto a Si-wafer were 100 and 200. By varying the number of layers, a difference in propulsion velocity/rotation comparable to depositing double the amount of material can be found. In order to have well-defined structures during inkjet printing, it is important that the correct jetting parameters are selected in order to achieve a well-defined droplet as shown in Figure 2. These parameters will vary according to the ink used and the jetting devices. If the ink does not produce stable droplets, then the ink is most likely no longer suitable for printing and is most likely starting to turn into gel. It is important to note that the resolution limit strongly depends on the size of nozzle used, and smaller nozzles allow for higher resolution and smaller structures/particles being printed.
An example of RIJ printed silk stirrer is shown in Figure 4, where the catalase doped engine part (as indicated by the red marked region) can be seen attached to the side of the main body (also see the schematic in Figure 1 for details). The printed silk scaffold is a material that allows for the fuel solution to diffuse throughout the entire 3D structure, and thus oxygen bubbles are formed during the decomposition of hydrogen peroxide via catalase. The oxygen bubbles being released generate micron-scale pores in the silk scaffold structure as can be seen by comparing the SEM micrographs before exposure to H2O2 fuel (Figure 5A) and after H2O2 exposure (Figure 5B). In order to ensure that motion is due to the decomposition of H2O2 fuel but not surface tension driven via the release of PEG400, it is important that stirrers are initially immersed in water for a period of at least 10 min and tested in DI water for surface tension motion prior to propulsion in fuel solution.
The use of PEG400 allows for better release of bubbles from the silk surface19 as previously explained by Gregory et al.5 but can also give rise to surface tension driven propulsion, which may be desirable depending on the application as previously described20. This second mechanism also gives the opportunity to produce SPMSs with two mechanisms that are time dependant and can be advantageous for certain applications that would for example expect initial vigorous stirring at the start followed by slower continued stirring for extended periods of time20.
In conclusion, by using RIJ to produce autonomous self-propelled devices, a vast range of shapes and sizes can be easily designed and printed. Silk as a base material for the devices gives opportunities to easily encapsulate enzymes and other moieties into the structures giving the possibility to add functions to these devices.

Artificial self-propelled micro-stirrers (SPMSs) employ a variety of propulsion mechanisms to produce motion, which can be categorized as either chemical propulsion1,2,3,4,5,6 or physical external propulsion. A common chemical propulsion mechanism is to use catalytic or enzymatic activity to either generate motion producing gradients or generate bubbles that impart momentum to the object when they detach. Previous studies have investigated several catalytic and chemical SPMSs, including polystyrene beads with platinum nanoparticles and chromium adsorbed on the surface1, gold-platinum bimetallic Janus nano-rods2, magnesium Janus micro-stirrers3, micro-stirrers made of a magnesium core and titanium dioxide shell with embedded gold nanoparticles4, and silk fibroin Janus micro-rockets with catalase embedded within the scaffold5. Physical propulsion mechanisms include magnetic7,8, optical9, and ultrasonic10 propulsion systems, all being controlled by an external physical source. Depending on the intended application, SPMS size can range from a few nanometers to several hundreds of micrometers. Examples of potential applications of these abovementioned and other SPMSs are medical diagnosis of diseases with lab-on-a-chip devices11, loading and in vivo targeted delivery of therapeutics12, environmental remediation3 (e.g., cleaning oil spills), and photocatalytic degradation of chemical and biological warfare agents, such as Bacillus anthracis and nerve agents4. Target application dependent, it is therefore desirable to be able to produce SPMSs that undergo specific trajectories such as long linear trajectories for transport challenges or rotational trajectories for micro mixing applications. The focus here is on rotational motion for stirring applications.
There is no single established method to fabricate SPMSs, but for medical and environmental applications, it is essential to use a material that is biocompatible, biodegradable, eco-friendly, readily available, cheap and allows easy fabrication of complex SPMSs without requiring sophisticated equipment. Regenerated silk fibroin (RSF) is one such material that fulfils all these parameters along with being also approved by the Food and Drug Administration (FDA).
Silk is a generic term used for several naturally occurring fibrous proteins, of which, the most commonly known is the one made by the larvae of silk moth, Bombyx mori, before its pupation. These cocoons are made of fibroin, a fibrous protein, stuck together with another protein called sericin. Silk fibroin (SF) has been found to have excellent mechanical properties, biocompatibility, and biodegradability13, which makes it an ideal choice for fabricating SPMSs. SF exists in three polymorphic forms, namely, silk I, II, and III. Silk I is a water-soluble metastable form containing mainly helixes and random coils; silk II is a water-insoluble form containing mainly antiparallel &#946; sheets of crystallized silk; and silk III is a threefold polyglycine II helical structure that exists at the water-air interface of the silk solution. Similar to other fibrous proteins, SF has repeating units of amino acid sequences. The naturally occurring SF of a cocoon consists of three main hexapeptide domains of such repeating units (GAGAGX), while X can be A, S, or Y. Through hydrogen binding, the (GA)n motifs from antiparallel &#946; sheet structures further stack through van der Waals forces and form hydrophobic nano crystallizations14,15.
Biocompatibility can be further improved by seeking to drive propulsion using naturally occurring enzymes to generate a concentration gradient or gas bubbles in a liquid medium (fuel). Consequently, in this study, the enzyme catalase is used as the &#34;engine&#34; to generate propulsion with hydrogen peroxide being used as the aqueous fuel medium. Catalase is an enzyme found in almost all living organisms. It catalyzes the decomposition of hydrogen peroxide (H2O2) into water and oxygen16. The release of oxygen bubbles from the enzyme sites of the SPMSs generates a force onto the object causing it to undergo propulsion in the opposite direction of the bubble release5 (Figure 1).
In a catalytically driven SPMS, different positioning of the catalytic site results in different propulsion behaviour and trajectories1. In pursuit of generating efficient micro-stirrers, it is therefore, necessary to fabricate stirrers with well-defined geometric shapes and engine positions and compare the different powers of the engine. Here, to facilitate these investigations, it is described how regenerated silk fibroin SPMSs are fabricated at millimeter scale using reactive inkjet printing (RIJ) technology. Inkjet printing is a non-contact method for the deposition of materials. This makes the fabrication of small complex structures with high precision in generating various shapes straightforward. RIJ takes place when two or more different reacting materials are deposited and react on the substrate to produce the desired product material. Therefore, SPMSs printed with one catalytic stirrer site off center gives the object asymmetry that results in rotational motion. This approach also makes it simple to manufacture micro-stirrers in a variety of shapes and design configurations defined by computer-aided design (CAD), thus allowing easier and more accurate controllability on the desired movement during practical applications. Finally, the ability of printing devices with varying thickness that exhibit different propulsion properties is demonstrated.
This study provides a blueprint for manufacturing SPMSs with RSF at micrometer to millimeter scale. The use of RIJ technology for manufacturing RSF micro-stirrers opens the door for the highly versatile production of micro-stirrers from materials such as in situ produced scaffolds or hydrogels, which are not otherwise able to be deposited or fabricated through other means such as evaporation. After appropriate further functionalization (e.g., enzymes), these SPMSs can be suitable for environmental remediation3, such as cleaning biological toxins, organic pollutants, and chemical and biological warfare agents4.

<table border="0" cellpadding="0" cellspacing="0" style="width:944px;" width="943">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium Carbonate</td>
			<td style="width:277px;">Alfa Aesar</td>
			<td align="right" style="width:140px;">11552</td>
			<td style="width:311px;">anhydrous, 99.5%, granular</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Calcium Chloride</td>
			<td style="width:277px;">Fluka Analytical</td>
			<td>C1016</td>
			<td>anhydrous, &#62;93%, granular</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ethanol</td>
			<td style="width:277px;">Fisher Scientific</td>
			<td align="right" style="width:140px;">10542382</td>
			<td>HPLC grade</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">PEG-400</td>
			<td style="width:277px;">Aldrich Chemistry</td>
			<td align="right" style="width:140px;">202398</td>
			<td>average Mn 400, tetramer mol wt ~250 kDa&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Catalase</td>
			<td style="width:277px;">Sigma Life Science</td>
			<td style="width:140px;">E3289</td>
			<td>&#62;20K units</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Methanol</td>
			<td style="width:277px;">Acros Organics</td>
			<td align="right" style="width:140px;">268280025</td>
			<td>HPLC grade</td>
		</tr>
		<tr height="27">
			<td height="27" style="height:27px;width:216px;">Hydrogen Peroxide</td>
			<td style="width:277px;">Sigma Aldrich</td>
			<td align="right" style="width:140px;">31642</td>
			<td>30% (w/w)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Silk</td>
			<td style="width:277px;">Southwest University, China</td>
			<td style="width:140px;">NA</td>
			<td>Raw Cleaned Silk Cocoons, Bombox Mori</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Dialysis Tubes</td>
			<td style="width:277px;">Sigma Aldrich</td>
			<td style="width:140px;">D9777</td>
			<td style="width:311px;">Cellulose, avg, flat width 25 mm, Typical molecular weight cut-off = 14000</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Fisherbrand Hoffman Clips&#160;</td>
			<td style="width:277px;">Fisher Scientific</td>
			<td align="right">12744396</td>
			<td style="width:311px;">Clips used to close the ends of the dialysis tubes</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Si-Wafer</td>
			<td style="width:277px;">Sigma Aldrich</td>
			<td align="right">647535</td>
			<td style="width:311px;">Used as printing substrate</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Balance</td>
			<td style="width:277px;">OHAUS Pioneer&#160;</td>
			<td style="width:140px;">PA214C&#160;</td>
			<td>Analytical Balance</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Conductivity meter</td>
			<td style="width:277px;">Mettler Toledo</td>
			<td>FG3</td>
			<td style="width:311px;">Mettler Toledo FiveGo Portable conductivity meter</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Centrifuge</td>
			<td>Thermo Scientific</td>
			<td>10355052</td>
			<td style="width:311px;">Heraeus Biofuge fresco sold by Thermo Scientific</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hotplate</td>
			<td style="width:277px;">Stuart</td>
			<td style="width:140px;">US152</td>
			<td>Stuart US152 Magnetic Stirrer</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Camera</td>
			<td style="width:277px;">PixeLink</td>
			<td style="width:140px;">PL-D732CU-T</td>
			<td>High Speed Colour Camera</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Lens</td>
			<td style="width:277px;">Navitar</td>
			<td style="width:140px;">Navitar 1-60135</td>
			<td>Macro Zoom Lens</td>
		</tr>
		<tr height="21">
			<td height="42" rowspan="2" style="height:42px;width:216px;">Jetting Devices</td>
			<td rowspan="2" style="width:277px;">Microfab Technologies Inc.</td>
			<td style="width:140px;">MJ-AT-01-40-8MX</td>
			<td>80um nozzle diameter Jetting device</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:140px;">MJ-AT-01-80-8MX</td>
			<td>80um nozzle diameter Jetting device</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Lightpad</td>
			<td style="width:277px;">AGPTEK</td>
			<td style="width:140px;">UN-HL0245-EUUN</td>
			<td>Light for the swimming experiment</td>
		</tr>
		<tr height="21">
			<td height="42" rowspan="2" style="height:42px;width:216px;">Pipettors</td>
			<td rowspan="2" style="width:277px;">Eppendorf</td>
			<td>3123000063</td>
			<td>single-channel, variable, &#160;100&#160;&#8211;&#160;1,000&#160;&#181;L, blue</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3123000055</td>
			<td>single-channel, variable, 20&#160;&#8211;&#160;200&#160;&#181;L, yellow</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Microscope</td>
			<td style="width:277px;">Nikon</td>
			<td>LV100ND</td>
			<td>Manual, upright microscope</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">SEM</td>
			<td style="width:277px;">Fei</td>
			<td style="width:140px;">F50</td>
			<td>Used for Scanning electron micrographs</td>
		</tr>
	</tbody>
</table>

reactive inkjet printing and propulsion analysis of silk-based self-propelled micro-stirrers

In this study, a protocol for using reactive inkjet printing to fabricate enzymatically propelled silk swimmers with well-defined shapes is reported. The resulting devices are an example of self-propelled objects capable of generating motion without external actuation and have potential applications in medicine and environmental sciences for a variety of purposes ranging from micro-stirring, targeted therapeutic delivery, to water remediation (e.g., cleaning oil spills). This method employs reactive inkjet printing to generate well-defined small-scale solid silk structures by converting water soluble regenerated silk fibroin (silk I) to insoluble silk fibroin (silk II). These structures are also selectively doped in specific regions with the enzyme catalase in order to produce motion via bubble generation and detachment. The number of layers printed determines the three-dimensional (3D) structure of the device, and so here the effect of this parameter on the propulsive trajectories is reported. The results demonstrate the ability to tune the motion by varying the dimensions of the printed structures.

After boiling the silk, it is expected that the dried fibers are around one third lighter than before, indicating the successful removal of sericin. During the dissolution of the silk in Ajisawa&#39;s reagent, the fibers should be completely dissolved, and a yellow viscous liquid should be recovered. After dialysis, the silk solution should be less viscous but still show a slightly yellow color. If the silk has turned into gel, this indicates that dissolution was not done successfully.
Stable droplets formed from the jetting devices will enable the higher definition of the printed samples. Figure 2 shows an example of a stable single droplet to give good printing results such as the printed silk-stirrers shown in Figure 4. It is normal, depending how viscous the ink is, that spreading occurs on the substrate.
Depending on the inkjet printers used and the droplet size, the distance between each printed droplet needs to be adjusted in such a way that they overlap to generate connected lines. If the droplets are too far apart, the printed structure will break up. In addition to this, if not enough layers are printed, there is a chance of the micro-stirrers breaking when placed into fuel solution. Once the stirrers have been removed off the substrate and washed, placing them into the hydrogen peroxide fuel solution should instantly result in bubbles being formed. The success rate of good bubble release depends strongly on the enzyme activity; if the enzyme activity is low, fewer bubbles will form thus leading to poor propulsion results. Figure 5 shows how the surface morphology of the stirrers is altered due to the bubbles being released from the inner structures generating small pores. A successful micro-stirrer will look similar to the ones that can be seen in Figure 6 and the two Supplemental Videos S1 and S2 respectively.
Figure 6 shows still video frames of two representative, 100-layer (Figure 6A) and 200-layer (Figure 6B) micro-stirrers in 5% H2O2 fuel. The red and green lines indicate the trajectories tracked (see Supplemental Videos S1 and S2). The rotational velocity can be determined by the rate of change of orientation (&#632;, Figure 3) as shown in Figure 7. Comparison of 100-layer and 200-layer catalase doped micro-stirrers shows a distinctive increase in rotational velocity of ~0.6 fold from 60 &#177; 6 rpm to 100 &#177; 10 rpm (Figure 7).
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/59030/59030fig1v2.jpg" /> 
Figure 1: Schematic illustration of the catalytic breakdown of hydrogen peroxide into water and oxygen by catalase embedded in the stirrer&#39;s scaffold at desired locations (shown in red). The product oxygen bubbles provide the necessary propulsion for moving the stirrer. <a href="https://www.jove.com/files/ftp_upload/59030/59030fig1v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/59030/59030fig2v2.jpg" /> 
Figure 2: The time lapse images of the droplet formation of RSF from a jetting device (nozzle diameter 80 &#181;m). The numbers below the images represent the time elapsed, in microseconds (&#181;s), since the initiation of jetting of the silk ink droplet. <a href="https://www.jove.com/files/ftp_upload/59030/59030fig2v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/59030/59030fig3.jpg" /> 
Figure 3: Schematic representation of particle tracking over two consecutive frames. A and B indicate tracking points and C indicates the center of mass.&#160;&#966; indicates the angle of orientation. SPMS trajectory direction is indicated by the curved black arrow. <a href="https://www.jove.com/files/ftp_upload/59030/59030fig3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/59030/59030fig4.jpg" /> 
Figure 4: Light micrograph of a freshly RIJ printed micro-stirrer (100 layers) before washing. Red box denotes catalase doped region (engine region). <a href="https://www.jove.com/files/ftp_upload/59030/59030fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/59030/59030fig5.jpg" /> 
Figure 5: SEM images of the main body and catalase engine part of a SPMS after pores are formed due to bubble release. Pores can be clearly seen on the engine surface in the SEM images of the SPMSs originating from the oxygen bubble release. (A) Silk micro-stirrers before exposure to 5% w/v H2O2 fuel solution. (B) Silk SPMS after exposure to 5% w/v H2O2 fuel solution. Images on the right are enlargements of the red regions. <a href="https://www.jove.com/files/ftp_upload/59030/59030fig5large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 6" class="xfigimg" src="/files/ftp_upload/59030/59030fig6.jpg" /> 
Figure 6: Video frames of two micro-stirrers in 5% fuel solution showing the trajectory over time. (A) 100-layer micro-stirrers. (B) 200-layer micro-stirrers. <a href="https://www.jove.com/files/ftp_upload/59030/59030fig6large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 7" class="xfigimg" src="/files/ftp_upload/59030/59030fig7.jpg" /> 
Figure 7: Comparison of angle of orientation (&#966;) for 100-layer (60 &#177; 6 rpm) and 200-layer (100 &#177; 10 rpm) micro-stirrers. <a href="https://www.jove.com/files/ftp_upload/59030/59030fig7large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Supplemental Video S1: Representative 100-layer self-motile micro-stirrer in 5% w/v H2O2 undergoing propulsion. <a href="https://www.jove.com/files/ftp_upload/59030/Video_1.mp4">Please click here to download this file.</a>
Supplemental Video S2: Representative 200-layer self-motile micro-stirrer in 5% w/v H2O2 undergoing propulsion. <a href="https://www.jove.com/files/ftp_upload/59030/Video_2.mp4">Please click here to download this file.</a>

Watch this Scientific Journal Video about Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers at JoVE.com

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers

Impressão de jato de tinta reativa e análise de propulsão de micro-agitadores autopropulsionados à base de seda

This protocol demonstrates the ability to utilize reactive inkjet printing to print self-motile biocompatible and environmentally friendly micro-stirrers for use in biomedical and environmental applications.

In this study, a protocol for using reactive inkjet printing to fabricate enzymatically propelled silk swimmers with well-defined shapes is reported. The ...

reactive-inkjet-printing-propulsion-analysis-silk-based-self

Research

JoVE Journal

Chemistry

7.7K visualizações.  University of Sheffield. Somos capazes de produzir micromotores de seda versáteis, biocompatíveis e biodegradáveis para uso em aplicações de micro-mistura, como aprimoramento de ensaios de enzimas, diagnósticos de cavacos de madeira de lava e monitoramento e remediação ambiental. Utilizar a impressão de jato de tinta reativa para criar micro-agitadores de seda alimentados por enzimas e marangoni nos permite alterar formas, tamanhos, distribuições de catalisadores e misturar outras moieties nas estruturas d...

Vídeo: Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials

Supercritical fluid extraction and drying methods are well established in numerous applications for the synthesis and processing of porous materials. Herein, nitrogen is presented as a novel supercritical drying fluid for specialized applications such as in the processing of reactive porous materials, where carbon dioxide and other fluids are not appropriate due to their higher chemical reactivity. Nitrogen exhibits similar physical properties in the near-critical region of its phase diagram as compared to carbon dioxide: a widely tunable density up to ~1 g ml-1, modest critical pressure (3.4 MPa), and small molecular diameter of ~3.6 &#197;. The key to achieving a high solvation power of nitrogen is to apply a processing temperature in the range of 80-150 K, where the density of nitrogen is an order of magnitude higher than at similar pressures near ambient temperature. The detailed solvation properties of nitrogen, and especially its selectivity, across a wide range of common target species of extraction still require further investigation. Herein we describe a protocol for the supercritical nitrogen processing of porous magnesium borohydride.

Nitrogen is an effective supercritical fluid for extraction or drying processes due to its small molecular size, high density in the near-liquid supercritical regime, and chemical inertness. We present a supercritical nitrogen drying protocol for the purification treatment of reactive, porous materials.

Processamento de nitrogênio supercrítico para a purificação de Reativos Porous Materials

Supercritical fluid extraction and drying methods are well established in numerous applications for the synthesis and processing of porous materials. ...

Química

Chemical Gardens as Flow-through Reactors Simulating Natural Hydrothermal Systems

Here we report experimental simulations of hydrothermal chimney growth using injection chemical garden methods. The versatility of this type of experiment allows for testing of various proposed ocean / hydrothermal fluid chemistries that could have driven reactions toward the origin of life in environments on the early Earth, early Mars, or even other worlds such as the icy moons of the outer planets. We show experiments that include growth of chemical garden structures under anoxic conditions simulating the early Earth, inclusion of trace components of phosphates / organics in the injection solution to incorporate them into the structure, a switch of the injection solution to introduce a secondary precipitating anion, and the measurement of membrane potentials generated by chemical gardens. Using this method, self-assembling chemical garden structures were formed that mimic the natural chimneys precipitated at submarine hydrothermal springs, and these precipitates can be used successfully as flow-through reactors by feeding through multiple successive &#8220;hydrothermal&#8221; injections.

We describe chemical garden formation via injection experiments that allow for laboratory simulations of natural chemical garden systems that form at submarine hydrothermal vents.

Jardins químicos como Fluxo através Reatores simulando Naturais sistemas hidrotérmicos

Here we report experimental simulations of hydrothermal chimney growth using injection chemical garden methods. The versatility of this type of experiment ...

Reactive Vapor Deposition of Conjugated Polymer Films on Arbitrary Substrates

We demonstrate a method of conformally coating conjugated polymers on arbitrary substrates using a custom-designed, low-pressure reaction chamber. Conductive polymers, poly(3,4-ethylenedioxythiophene) (PEDOT) and poly(3,4-propylenedioxythiophene) (PProDOT), and a semiconducting polymer, poly(thieno[3,2-b]thiophene) (PTT), were deposited on unconventional highly-disordered and textured substrates with high surface areas, such as paper, towels and fabrics. This reported deposition chamber is an improvement of previous vapor reactors because our system can accommodate both volatile and nonvolatile monomers, such as 3,4-propylenedioxythiophene and thieno[3,2-b]thiophene. Utilization of both solid and liquid oxidants are also demonstrated. One limitation of this method is that it lacks sophisticated in situ thickness monitors. Polymer coatings made by the commonly used solution-based coating methods, such as spin-coating and surface grafting, are often not uniform or susceptible to mechanical degradation. This reported vapor phase deposition method overcomes those drawbacks and is a strong alternative to common solution-based coating methods. Notably, polymer films coated by the reported method are uniform and conformal on rough surfaces, even at a micrometer scale. This feature allows for future application of vapor deposited polymers in electronics devices on flexible and highly textured substrates.

This paper presents a protocol for reactive vapor deposition of poly(3,4-ethylenedioxythiophene), poly(3,4-propylenedioxythiophene), and poly(thieno[3,2-b]thiophene) films on glass slides and rough substrates, such as textiles and paper.

Depósito de Vapor reativa dos filmes de polímeros conjugados em substratos arbitrários

We demonstrate a method of conformally coating conjugated polymers on arbitrary substrates using a custom-designed, low-pressure reaction chamber. ...

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol

The purification of organic compounds is an essential component of routine synthetic operations. The ability to remove contaminants into an aqueous layer by generating a charged structure provides an opportunity to use extraction as a simple purification technique. By combining the use of a miscible organic solvent with saturated sodium bisulfite, aldehydes and reactive ketones can be successfully transformed into charged bisulfite adducts that can then be separated from other organic components of a mixture by the introduction of an immiscible organic layer. Here, we describe a simple protocol for the removal of aldehydes, including sterically-hindered neopentyl aldehydes and some ketones, from chemical mixtures. Ketones can be separated if they are sterically unhindered cyclic or methyl ketones. For aliphatic aldehydes and ketones, dimethylformamide is used as the miscible solvent to improve removal rates. The bisulfite addition reaction can be reversed by basification of the aqueous layer, allowing for the re-isolation of the reactive carbonyl component of a mixture.

Here, we present a protocol to remove aldehydes and reactive ketones from mixtures by a liquid-liquid extraction protocol directly with saturated sodium bisulfite in a miscible solvent. This combined protocol is rapid and facile to perform. The aldehyde or ketone can be re-isolated by the basification of the aqueous layer.

Separação de aldeídos e cetonas reativas de misturas usando um protocolo de extração de bissulfito

The purification of organic compounds is an essential component of routine synthetic operations. The ability to remove contaminants into an aqueous layer ...

Stereolithographic 3D Printing with Renewable Acrylates

The accessibility of cost-competitive renewable materials and their application in additive manufacturing is essential for an efficient biobased economy. We demonstrate the rapid prototyping of sustainable resins using a stereolithographic 3D printer. Resin formulation takes place by straightforward mixing of biobased acrylate monomers and oligomers with a photoinitiatior and optical absorber. Resin viscosity is controlled by the monomer to oligomer ratio and is determined as a function of shear rate by a rheometer with parallel plate geometry. A stereolithographic apparatus charged with the biobased resins is employed to produce complex shaped prototypes with high accuracy. The products require a post-treatment, including alcohol rinsing and UV irradiation, to ensure complete curing. The high feature resolution and excellent surface finishing of the prototypes is revealed by scanning electron microscopy.

A protocol for additive manufacturing with renewable photopolymer resins on a stereolithography apparatus is presented.

Stereolithographic de impressão 3D com acrilatos renováveis

The accessibility of cost-competitive renewable materials and their application in additive manufacturing is essential for an efficient biobased economy. ...

Inkjet Printing All Inorganic Halide Perovskite Inks for Photovoltaic Applications

A method for synthesizing photoactive inorganic&#160;perovskite quantum dot inks and an inkjet printer deposition method, using the synthesized inks, are demonstrated. The ink synthesis is based on a simple wet chemical reaction and the inkjet printing protocol is a facile step by step method. The inkjet printed thin films have been characterized by X-ray diffraction, optical absorption spectroscopy, photoluminescent spectroscopy, and electronic transport measurements. X-ray diffraction of the printed quantum dot films indicates a crystal structure consistent with an orthorhombic room temperature phase with (001) orientation. In conjunction with other characterization methods, the X-ray diffraction&#160;measurements show high quality films can be obtained through the inkjet printing method.

A protocol for synthesizing inorganic-lead-halide hybrid perovskite quantum dot inks for inkjet printing and the protocol for preparing and printing the quantum dot inks in an inkjet printer with post characterization techniques are presented.

Jato de tinta de impressão de todas as tintas de perovskita iodetos inorgânicos para aplicações fotovoltaicas

A method for synthesizing photoactive inorganic&#160;perovskite quantum dot inks and an inkjet printer deposition method, using the synthesized inks, are ...

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Maleimide-bearing bifunctional probes have been employed for decades for the site-selective modification of thiols in biomolecules, especially antibodies. Yet maleimide-based conjugates display limited stability in vivo because the succinimidyl thioether linkage can undergo a retro-Michael reaction. This, of course, can lead to the release of the radioactive payload or its exchange with thiol-bearing biomolecules in circulation. Both of these processes can produce elevated activity concentrations in healthy organs as well as decreased activity concentrations in target tissues, resulting in reduced imaging contrast and lower therapeutic ratios. In 2018, we reported the creation of a modular, stable, and easily accessible phenyloxadiazolyl methyl sulfone reagent &#8212; dubbed &#8216;PODS&#8217; &#8212; as a platform for thiol-based bioconjugations. We have clearly demonstrated that PODS-based site-selective bioconjugations reproducibly and robustly create homogenous, well-defined, highly immunoreactive, and highly stable radioimmunoconjugates. Furthermore, preclinical experiments in murine models of colorectal cancer have shown that these site-selectively labeled radioimmunoconjugates exhibit far superior in vivo performance compared to radiolabeled antibodies synthesized via maleimide-based conjugations. In this protocol, we will describe the four-step synthesis of PODS, the creation of a bifunctional PODS-bearing variant of the ubiquitous chelator DOTA (PODS-DOTA), and the conjugation of PODS-DOTA to the HER2-targeting antibody trastuzumab.

In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.

Síntese e Bioconjugation dos reagentes Thiol-reativa para a criação de Site-seletivamente modificado Immunoconjugates

Maleimide-bearing bifunctional probes have been employed for decades for the site-selective modification of thiols in biomolecules, especially antibodies. ...

Niobium Oxide Films Deposited by Reactive Sputtering: Effect of Oxygen Flow Rate

Reactive sputtering is a versatile technique used to form compact films with excellent homogeneity. In addition, it allows easy control over deposition parameters such as gas flow rate that results in changes on composition and thus in the film required properties. In this report, reactive sputtering is used to deposit niobium oxide films. A niobium target is used as metal source and different oxygen flow rates to deposit niobium oxide films. The oxygen flow rate was changed from 3 to 10 sccm. The films deposited under low oxygen flow rates show higher electrical conductivity and provide better perovskite solar cells when used as electron transport layer.

Here, we present a protocol for niobium oxide films deposition by reactive sputtering with different oxygen flow rates for use as an electron transport layer in perovskite solar cells.

Óxido de nióbio filmes depositados por sputtering reativa: efeito da taxa de fluxo de oxigênio

Reactive sputtering is a versatile technique used to form compact films with excellent homogeneity. In addition, it allows easy control over deposition ...

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Despite its limited analytical specificity and ruggedness, the thiobarbituric acid reactive substances (TBARS) assay has been widely used as a generic metric of lipid peroxidation in biological fluids. It is often considered a good indicator of the levels of oxidative stress within a biological sample, provided that the sample has been properly handled and stored. The assay involves the reaction of lipid peroxidation products, primarily malondialdehyde (MDA), with thiobarbituric acid (TBA), which leads to the formation of MDA-TBA2 adducts called TBARS. TBARS yields a red-pink color that can be measured spectrophotometrically at 532 nm. The TBARS assay is performed under acidic conditions (pH = 4) and at 95 &#176;C. Pure MDA is unstable, but these conditions allow the release of MDA from MDA bis(dimethyl acetal), which is used as the analytical standard in this method. The TBARS assay is a straightforward method that can be completed in about 2 h. Preparation of assay reagents are described in detail here. Budget-conscious researchers can use these reagents for multiple experiments at a low cost rather than buying an expensive TBARS assay kit that only permits construction of a single standard curve (and thus can only be used for one experiment). The applicability of this TBARS assay is shown in human serum, low density lipoproteins, and cell lysates. The assay is consistent and reproducible, and limits of detection of 1.1 &#956;M can be reached. Recommendations for the use and interpretation of the spectrophotometric TBARS assay are provided.

The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

Avaliação do estresse oxidativo em amostras biológicas usando o ensaio de substâncias reativas de ácido tiobarbitúrico

Despite its limited analytical specificity and ruggedness, the thiobarbituric acid reactive substances (TBARS) assay has been widely used as a generic ...

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery

Due to drug resistance and toxicity in healthy cells, use of doxorubicin (DOX) has been limited in clinical cancer therapy. This protocol describes the designing of poly(ethylenimine) grafted with polyethylene glycol (PEI-g-PEG) copolymer functionalized gold nanoparticles (AuNPs) with loaded aptamer (AS1411) and DOX through amide reactions. AS1411 is specifically bonded with targeted nucleolin receptors on cancer cells so that DOX targets cancer cells instead of healthy cells. First, PEG is carboxylated, then grafted to branched PEI to obtain a PEI-g-PEG copolymer, which is confirmed by 1H NMR analysis. Next, PEI-g-PEG copolymer coated gold nanoparticles (PEI-g-PEG@AuNPs) are synthesized, and DOX and AS1411 are covalently bonded to AuNPs gradually via amide reactions. The diameter of the prepared AS1411-g-DOX-g-PEI-g-PEG@AuNPs is ~39.9 nm, with a zeta potential of -29.3 mV, indicating that the nanoparticles are stable in water and cell medium. Cell cytotoxicity assays show that the newly designed DOX loaded AuNPs are able to kill cancer cells (A549). This synthesis demonstrates the delicate arrangement of PEI-g-PEG copolymers, aptamers, and DOX on AuNPs that are achieved by sequential amide reactions. Such aptamer-PEI-g-PEG functionalized AuNPs provide a promising platform for targeted drug delivery in cancer therapy.

In this protocol, doxorubicin-loaded AS1411-g-PEI-g-PEG modified gold nanoparticles are synthesized via three-step amide reactions. Then, doxorubicin is loaded and delivered to target cancer cells for cancer therapy.

Síntese de Nanopartículas de ouro modificada Aptamer-PEI-g-PEG carregadas com doxorubicina para entrega de drogas direcionadas

Due to drug resistance and toxicity in healthy cells, use of doxorubicin (DOX) has been limited in clinical cancer therapy. This protocol describes the ...