Preparation of A Microfluidic Device in Polydimethylsiloxane (PDMS)
3:27
Preparation of a Fluidic Device in Poly(methyl methacrylate)(PMMA)
4:09
Analyze Bacterial Transport using PDMS Microfluidic Devices
5:10
Basic Image Processing
6:59
Analyze Bacterial Transport at the Pore Scale
8:08
Study Bacterial Filtration by Means of Deposition Profiles
8:51
Analyze Bacterial Transport using PMMA Fluidic Devices
10:33
Results: Motile and Nonmotile GFP-tagged P. putida KT2440
11:48
Conclusion
Transcrição
Studying the transport of bacteria in porous systems is important with regards to contamination, spread of disease, and bioremediation. For these experiments in mathematical models operating at the single cell, the population and the microbial com
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Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.