Nickel-Nitrilotriacetic Acid (Ni-NTA) Affinity Purification of Relseq and GppA
2:55
Gel Filtration
3:33
Synthesis of 32P-Labeled Guanosine Pentaphosphate (pppGpp) and Guanosine Tetraphosphate (ppGpp)
5:08
DRaCALA Screening of the Target Proteins of (p)ppGpp
6:37
Quantification and Identification of Potential Target Proteins
7:46
Results: DRaCALA Plate Screening and Quantification
8:39
Conclusion
TRANSCRIÇÃO
Small signaling molecules target various effector proteins to control bacterial virulence and human biology. However, these effector proteins are challenging to identify. As a systems biology tool, DRaCALA allows a feasible, rapid, and highly sens
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The Differential Radial Capillary Action of Ligand Assay (DRaCALA) can be used to identify small ligand binding proteins of an organism by using an ORFeome library.