Loading the Phylogenetic Codon Substitution Frequencies (PhyloCSF) Track Hub to the University of California Santa Cruz (UCSC) Genome Browser
1:25
Navigating to Genes of Interest Using Gene Identifiers
2:20
Navigating to Genomic Regions of Interest Using Sequence Information
3:10
Identifying Conserved Short Open Reading Frames (sORFs) Using PhyloCSF Track Data
4:06
Viewing Homologous Regions in Other Genomes
4:51
Generating Multi-Species Sequence Alignments for Microproteins of Interest
6:47
Results: Identification of Genomic Regions of Interest for Microprotein-Coding Potential
8:47
Conclusion
TRANSCRIÇÃO
The protocol described here provides detailed instructions on analyzing genomic regions of interest for protein-coding potential using phyloCSF on the user-friendly UCSC genome browser. PhloCSF can effectively identify conserved short open reading
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The protocol described here provides detailed instructions on how to analyze genomic regions of interest for microprotein-coding potential using PhyloCSF on the user-friendly UCSC Genome Browser. Additionally, several tools and resources are recommended to further investigate sequence characteristics of identified microproteins to gain insight into their putative functions.