Loading the Phylogenetic Codon Substitution Frequencies (PhyloCSF) Track Hub to the University of California Santa Cruz (UCSC) Genome Browser
1:25
Navigating to Genes of Interest Using Gene Identifiers
2:20
Navigating to Genomic Regions of Interest Using Sequence Information
3:10
Identifying Conserved Short Open Reading Frames (sORFs) Using PhyloCSF Track Data
4:06
Viewing Homologous Regions in Other Genomes
4:51
Generating Multi-Species Sequence Alignments for Microproteins of Interest
6:47
Results: Identification of Genomic Regions of Interest for Microprotein-Coding Potential
8:47
Conclusion
Transcrição
The protocol described here provides detailed instructions on analyzing genomic regions of interest for protein-coding potential using phyloCSF on the user-friendly UCSC genome browser. PhloCSF can effectively identify conserved short open reading
Faça login ou inicie seu teste gratuito para acessar este conteúdo
The protocol described here provides detailed instructions on how to analyze genomic regions of interest for microprotein-coding potential using PhyloCSF on the user-friendly UCSC Genome Browser. Additionally, several tools and resources are recommended to further investigate sequence characteristics of identified microproteins to gain insight into their putative functions.