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Abstract

Neuroscience

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Published: March 20th, 2019

DOI:

10.3791/59163

1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Abstract

In the nervous system, myelin is a complex membrane structure generated by myelinating glial cells, which ensheathes axons and facilitates fast electrical conduction. Myelin alteration has been shown to occur in various neurological diseases, where it is associated with functional deficits. Here, we provide a detailed description of an ex vivo model consisting of mouse organotypic cerebellar slices, which can be maintained in culture for several weeks and further be labeled to visualize myelin.

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Keywords Myelinating Organotypic Cerebellar Slice Cultures

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