A Training and Testing System for Performing Vascular Reconstruction In Vitro

Summary

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.

Abstract

Manual vascular reconstruction training is essential for a beginner surgeon. However, an optimal training system for vascular reconstruction in vitro has yet to be developed. In this study, we introduce an in vitro training and testing system using a magnetic anchoring technique with which a trainee can practice manual vascular reconstruction individually. Additionally, this system can also be used to test the quality of the reconstruction. The described system includes a vascular reconstruction training machine, magnetic tractors, and a magnetic suture puller. In this manuscript, we detail an end-to-end vein anastomosis using porcine right and left iliac veins. To identify the potential damage caused by a magnetic suture puller on the suture, we created three groups with six segments of 4-0 polypropylene sutures each: a control group with no intervention on the polypropylene suture, a group in which the polypropylene suture is manually pulled with sterile gloves 20x, and a magnetic puller group in which the magnetic puller pulled the polypropylene suture 20x. These groups were tested by light microscopy and breaking strength tests, and the effect of reconstruction was assessed. In the light microscopy test, the control group was less likely to be damaged (p < 0.05) and the number of damaged points of the manual group and magnetic puller group were similar (p > 0.05). The results of the breaking strength test were compared across groups and no significant difference was observed (p > 0.05). The end-to-end anastomosis of the porcine iliac veins was successfully performed using this training system, and the reconstructed veins could undergo 2.0 kPa perfusion pressure. Using this training and testing system the trainee can practice manual vascular reconstruction in vitro individually with the aid of magnetic tractors and a magnetic suture puller, and the quality of the reconstruction can be tested.

Introduction

Vascular reconstruction is a basic skill required for surgeons. Although Obora¹ and Holt² invented several mechanical reconstruction methods to simplify the reconstruction of small vessels (diameters <10 mm), these methods are not commonly applied in macrovascular anastomosis. Manual vascular anastomosis is still performed in many operations, including vascular surgery³, emergency surgery⁴, and solid organ transplantation⁵. Thus, it is essential for surgeons to practice manual vascular anastomosis. However, an optimal training system for vascular reconstruction in vitro is uncommon, and inexperienced surgeons must undergo considerable training in vivo on large animals⁶ before they can master the technique. Because failure is inevitable during initial training, many animals are likely to die of vascular complications, which is concerning regarding animal welfare. Further, during the procedure of end-to-end vascular reconstruction, to avoid mistakes in stitch positions or loose sutures, the surgeon needs at least one assistant to expose the posterior vascular wall and pull the suture. Thus, vascular reconstruction usually cannot be performed by the surgeon individually, and the efficiency of preparation is usually limited by the proficiency of the assistant.

Magnetic anchoring surgery has become a topic of interest in recent years^7,8,9,10,11. The clinical trial by Rivas et al.⁷ showed that with his magnetic surgical instrument and following the principle of magnetic anchoring, surgeons can perform reduced-port laparoscopic cholecystectomy. The use of this instrument also allows for a reduced role for the assistant during open surgery. Through the magnetic field, the magnetic device is adsorbed onto an anchoring point. This magnetic anchoring device can act as a mechanical arm, grasping and retracting the tissue or organ, exposing the surgical field, and simplifying the operation. Based on this rationale, we invented magnetic tractors to retract the vascular wall and suture, and a magnetic suture puller to pull the polypropylene sutures.

The use of a vascular reconstruction training machine was another milestone in this study. It consists of an operating floor and a control panel: the vasculature is fixed on the operating floor, and the trainee can practice on it. After anastomosis, the trainee can set the perfusion parameters on the control panel in order to test the quality of anastomosis. Compared to previous vascular anastomosis training systems^6,12,13,14, the use of this system provides two main advantages: First, magnetic devices can be used to expose the surgical field, so that the trainees can practice on it individually. Second, the trainee can check the effect of anastomosis using a perfusion test.

In the present study, we introduce a training and testing system where the trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique and the quality of reconstruction can also be tested. Limited by the design and size of the water inlet and water outlet on the operating floor, the training system can only perform end-to-end reconstruction on vessels with a >5 mm diameter.

Protocol

The protocol was carried out in accordance with the Guidelines for the Care and Use of Laboratory Animals and was approved by the Committee on the Ethics of Animal Experiments of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

1. Preparation prior to the training

NOTE: The vascular reconstruction training machine is shown in Figure 1. It consists of a control panel and an operating floor.

1. Click the Clean button on the control panel to clean and drain the residual liquid from the operating floor.
2. Click the Add Liquid button on the control panel and add 0.9% saline into the machine from the operating floor until the prompt "The testing liquid is adequate" appears on the control panel.
3. Prepare the magnetic tractor, which consists of a circular permanent magnet with a diameter of 20 mm and a thickness of 1 mm, an acrylonitrile butadiene styrene (ABS) plastic casing, a spiral spring, a 30 cm nylon traction wire, and a stainless-steel clamp with plastic sleeves or a vascular clamp.
   1. Glue the circular magnet and the plastic casing together using an acrylate adhesive. The traction force will increase with the lengthening of the traction wire. Use a universal testing machine to test the association between the length of the traction wire and the traction force (Figure 2).
   2. Fix the clamp and the plastic casing on the upper holder and the lower holder of the universal testing machine, respectively. Gradually elevate the upper holder to stretch the traction wire between the two holders. Test the strength of the traction wire while it is being stretched.
      NOTE: The magnetic suture tractor and magnetic vascular tractor are shown in Figure 3.
4. Prepare a magnetic suture puller.
   1. Use a quasi-oval polylactic acid board with a thickness of 2 mm, a major axis diameter of 10 cm, a minor axis diameter of 2 cm, three magnetic balls with a diameter of 5 mm, and three magnetic cylinders with a diameter of 5 mm and a height of 5 mm.
   2. Punch three holes with a diameter of 3 mm and a depth of 0.5 mm on the polylactic acid board, so that the magnetic balls can cling to the board by magnetic attraction force from the magnetic cylinders under the board.
      NOTE: The magnetic suture puller is shown in Figure 4.
5. Fix the suture under the magnetic ball after one stitch. This plays the role of a suture puller, preventing the previous suture from loosening. Extract the end with the suture needle with a force of about 0.3 N, closely in parallel to the polylactic acid board, and continue the next stitch.
6. Ligate all the vein branches using 3-0 silk sutures to avoid leakage after anastomosis. Use tissue scissors to trim the ends of the veins and clear the excess tissue on the wall of the veins to make the veins smooth.
   NOTE: The vasculature used in this study included the right and left iliac veins (diameter ~10 mm) harvested from Bama pigs weighing 50–60 kg. To simplify the reconstruction, only a few branches of the iliac veins were picked, and the two veins were similar in size. The vasculature was kept at -20 °C. Before training, it was immersed in 0.9% saline at room temperature.

2. Fix the veins on the operating floor

1. Tie the two veins on the water inlet and water outlet of the training machine with 2-0 silk sutures.
   NOTE: This study uses the two-point vascular anastomosis⁵.
2. Adjust the length of the water outlet of the training machine and ensure that the ends of the two veins are tension-free in a parallel direction.
3. Straighten the veins and lay two 4-0 polypropylene traction sutures at the 6 o’clock and 12 o’clock positions.
4. Insert the needle of the traction sutures from the outside of the vein and then insert from the inside of the other vein.
5. Wet the surgical glove and sutures to avoid damaging the sutures. Gently tie at least five knots to avoid tearing the walls of the veins.
6. Use the two stainless-steel clamps of the magnetic suture tractor to grasp the traction sutures and attract the circular magnets of the magnetic suture tractors to the ferromagnetic stainless-steel operating floor. Adjust the position of magnetic attraction and ensure that the ends of the two veins are stretched in a vertical direction.
7. Use the two vascular clamps of the magnetic vascular tractor to clamp the anterior wall of the veins and attract the circular magnets of the magnetic vascular tractors on the operating floor. Adjust the position of attraction and ensure that the anterior walls of the veins are retracted, and the posterior walls of the veins are clearly exposed.

3. Anastomosis of posterior walls

1. Use the two stainless-steel clamps of the magnetic suture tractor to grasp the traction sutures and attract the circular magnets of the magnetic suture tractors on the ferromagnetic stainless-steel operating floor. Leave the tail segment of the polypropylene suture at the 12 o’clock position for the traction suture and use the segment with the needle for continuous suture.
2. Ensure intima-to-intima contact between the two veins.
3. Insert the first suture from the outside of the vein to the inside.
4. In subsequent sutures, insert the needle from the inside of the vein and then insert from the outside of the other vein.
5. Check that the sutures are not loose.
6. After one suture, ensure that the polypropylene suture is hung on the magnetic suture puller and pull the polypropylene gently until the magnetic ball presses the polypropylene.
7. Extract the end with the needle of the suture with a force of about 0.3 N, closely in parallel to the polylactic acid board, and continue the next stitch.
   NOTE: By using this technique, the tail of the polypropylene suture will be sufficiently tight. As the sutures continue, the polypropylene suture will become shorter. According to the length of the suture, select the most suitable of the three magnetic balls, and then manually press the suture under it.
8. Insert the last suture from the inside of the vein to the outside to ensure intima-to-intima contact between the two veins.
9. Avoid stenosis after anastomosis by two means: Keep same, proper margin and needle spacing when stitching, and keep the "growth factor"¹⁵ when knotting.
   NOTE: The "growth factor" is the reserved space away from the vessel wall when tying the first knot after the anastomosis so that vessels can remain flexible rather than stenose.
   1. Maintain the same suture margin and needle spacing.
      NOTE: In this study, iliac veins with a diameter of approximately 10 mm were used, so the suture margin and needle spacing was about 1 mm.
   2. Keep the "growth factor"¹⁵ when tying the knots. After anastomosis of the posterior walls, tie the end of the suture and the tail segment of the suture at the 6 o’clock position together away from the vein wall in order to prevent suture stenosis. Use the standard method for tying knots.

4. Anastomosis of anterior walls

1. After anastomosis of the posterior walls, remove the magnetic vascular tractor, leave the tail as a traction suture, and use the segment with the needle at the 6 o’clock position for the anastomosis of the anterior walls.
2. Insert the needle from the outside of the vein and then insert from the inside of the other vein.
   NOTE: The methods used in the anastomosis of the posterior walls to ensure the intima-to-intima contact between the two veins (the suture not being loose and avoiding stenosis after anastomosis) were followed in the anastomosis of the anterior walls^5,15.
3. After anastomosis of the anterior walls, cut off two traction sutures using suture scissors.

5. Test the effect of anastomosis

1. Set the test parameters.
   1. Set the perfusion pressure as 2.0 kPa on the control panel.
      NOTE: The normal vein pressure will not exceed 2.0 kPa.
   2. Set the duration of peak pressure as 5 s on the control panel.
   3. Set the temperature as 25 °C on the control panel.
   4. Set the pressure deviation as 0.1 kPa on the control panel.
2. Click the Test button and observe the time and pressure on the control panel and whether the reconstructed vein leaks.
   NOTE: If the vein does not leak during the peak pressure, the anastomosis is successful. If leaks are found, the leakage position should be located and sutured, and then the test should be performed again. The results of the test in this video are shown in Figure 5.

6. Checking the safety of the magnetic suture puller

NOTE: To test whether the magnetic suture puller damaged the polypropylene suture, perform the breaking strength and light microscopy tests. In this experiment, three groups with six segments of 4-0 polypropylene suture in each were tested: a control group with no intervention on the polypropylene suture, a manual group in which the polypropylene suture was manually pulled with sterile gloves 20x, and a magnetic puller group in which the magnetic puller pulled the polypropylene suture 20x.

1. Test the breaking strength of the polypropylene suture on the universal testing machine. Fix the two ends of the polypropylene suture on the upper holder and the lower holder of the universal testing machine. Gradually elevate the upper holder. Test the strength of the polypropylene suture while it is being stretched. Set the breaking strength as the tension force when the suture snaps. Compare the breaking strength among the three groups and perform pairwise comparisons.
2. Observe the damage of the polypropylene suture under a light microscope. Define the number of damage points as the number of fibrous or coarse fracture points visible at 200x magnification. Compare the number of damage points among the three groups and perform pairwise comparisons.

Results

The vascular reconstruction training machine is shown in Figure 1 and includes two main parts: the operating floor and the control panel. The operating floor consists of a water inlet, a water outlet, and a water storage basin. The two ends of the vasculature are tied to the water inlet and water outlet to test the effect of anastomosis. The length of the water outlet is adjustable, and we set the parameters (e.g., the perfusion pressure, duration of peak pressure, temperature, and pressure ...

Discussion

With the help of magnetic tractors and a magnetic suture puller, a trainee can complete vein anastomosis individually and precisely. Magnetic tractors pull the tissue that blocks the anastomosis field and provide suitable strength for stretching the veins in a vertical direction, thus achieving clear exposure for vein anastomosis. In traditional manual anastomosis, at least one assistant is required for surgical exposure. The use of magnetic tractors could achieve the required exposure and substitute for assistants. In a...

Materials

Name	Company	Catalog Number	Comments
Circular permanent magnet	Hangzhou Permanent Magnet Group Co.LTD	20*1mm	Magnetic tractor
Magnetic balls	Hangzhou Permanent Magnet Group Co.LTD	5mm	Magnetic suture puller
Magnetic cylinders	Hangzhou Permanent Magnet Group Co.LTD	5*5mm	Magnetic suture puller
Polypropylene suture	Johnson and Johnson	PROLENE 4-0	Used for anastomosis
Silk suture	SILK	2-0，3-0	Used for fixing vascular and ligation
Surgical insturments	Jinzhong Shanghai	JZ-2018	Suture scissors, tissue scissors， forceps, needle and needle holder
Universal testing machine	Zwick GmbH&Co	Z010	Used for testing the association between the length of traction wire and the traction force