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Abstract

Neuroscience

Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Published: December 18th, 2019

DOI:

10.3791/60353

1Department of Physiology, Northwestern University, Feinberg School of Medicine

Abstract

Organotypic slice culture is a powerful in vitro model that mimicks in vivo conditions more closely than dissociated primary cell cultures. In early postnatal development, cerebellar Purkinje cells are known to go through a vulnerable period, during which they undergo programmed cell death. Here, we provide a detailed protocol to perform mouse organotypic cerebellar slice culture during this critical time. The slices are further labeled to assess Purkinje cell survival and the efficacy of neuroprotective treatments. This method can be extremely valuable to screen for new neuroactive molecules.

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Keywords Purkinje Cell

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