Mechanical Control of Relaxation Using Intact Cardiac Trabeculae

Summary

Rapid myocardial and cardiac relaxation is essential for normal physiology. Mechanical relaxation mechanisms are now known to be dependent on strain rate. This protocol provides an overview of the acquisition and analysis of experiments to further study the mechanical control of relaxation.

Abstract

Diastolic dysfunction is a common phenotype across cardiovascular disease presentations. In addition to elevated cardiac stiffness (elevated left ventricular end-diastolic pressure), impaired cardiac relaxation is a key diagnostic indicator of diastolic dysfunction. While relaxation requires the removal of cytosolic calcium and deactivation of sarcomeric thin filaments, targeting such mechanisms has yet to provide effective treatments. Mechanical mechanisms, such as blood pressure (i.e., afterload), have been theorized to modify relaxation. Recently, we showed that modifying the strain rate of a stretch, not afterload, was both necessary and sufficient to modify the subsequent relaxation rate of myocardial tissue. The strain rate dependence of relaxation, called the mechanical control of relaxation (MCR), can be assessed using intact cardiac trabeculae. This protocol describes the preparation of a small animal model, experimental system and chamber, isolation of the heart and subsequent isolation of a trabecula, preparation of the experimental chamber, and experimental and analysis protocols. Evidence for lengthening strains in the intact heart suggests that MCR might provide new arenas for better characterization of pharmacological treatments, along with a method to assess myofilament kinetics in intact muscles. Therefore, studying the MCR may elucidate a path to novel approaches and new frontiers in the treatment of heart failure.

Introduction

Cardiac relaxation is impaired in nearly all forms of heart failure (including heart failure with reduced ejection fraction) and in many cardiovascular diseases. In addition to numerous methods to evaluate cardiac function in permeabilized muscles, the evaluation of intact cardiac muscles is gaining interest. Such tissues are assessed unloaded (ends free to contract) or loaded (length or force controlled). Historically, intact isolated myocytes have been evaluated in an unloaded condition, where the cell body is free to shorten during contraction. Intact cardiac trabeculae are often evaluated in isometric conditions, where the length is not allowed to change, but stress (force per cross-sectional area) is generated. Both intact myocyte and trabeculae methods are beginning to converge with modifications of load^1,2.

Protocols for load-clamping a muscle (i.e., controlling a muscle's developed stress at a specified value that simulates physiologic afterloads) have been developed over several decades^3,4,5. In intact cardiac tissues, load-clamps allow researchers to more closely mimic the in vivo cardiac cycle using isotonic or Windkessel-like afterloads^6,7,8,9. The goal of this protocol is to obtain data used to quantify the MCR (i.e., the strain rate dependence of the relaxation rate)^8,9.

While the MCR protocol has been adapted from prior work, the focus of this protocol (compared to similar protocols utilizing intact cardiac tissues) is on biomechanical mechanisms that modify relaxation. There are several protocols utilizing load-clamping^3,4,5,7,10 and protocols focusing on Windkessel models^1,2,11, but this protocol describes specifically how stretch prior to relaxation modifies the relaxation rate. We have shown that this control occurs during a proto-diastolic period⁸, a phase originally described by Wiggers¹². In normal healthy hearts, the myocardium undergoes a lengthening strain during ejection prior to aortic valve closure (i.e., prior to isovolumic relaxation)¹³. This is mimicked by prolonging the duration of afterload control until the muscle begins to stretch. Clinical evidence suggests that this lengthening may be attenuated or lost in disease states¹⁴, and the implications and mechanisms of altered end-systolic strain rates have not been fully elucidated. Given the sparse treatment options for diastolic diseases and heart failure with a preserved ejection fraction, we posit that MCR may provide insight into novel mechanisms underlying impaired relaxation.

While the gross dissection described here focuses on rodents, trabecula isolation can be performed from any intact heart, and has previously been used with a human cardiac trabecula⁸. Similarly, the data acquisition and analysis can also be applied to cardiomyocytes or other isolated muscle types^1,10. The discussion includes commentary on possible alterations and adaptations to the method, along with limitations, such as caution against utilizing papillary muscles because of the mechanical properties of the chordae⁹.

Protocol

The following protocol has been approved by the Institutional Animal Care and Use Committee of Wayne State University. The protocol here describes steps for utilizing rodent experimental subjects, but can be adapted for use in other model organisms.

1. Preparation

1. Obtain the experimental subject and allow it to acclimate to the lab.
2. Prepare 250 mL of perfusion solution and 250 mL of modified Tyrode's solution (Table 1), by oxygenating each to 10-20 ppm of O₂, with pH 7.3.
3. Prepare a 5 mL syringe with a cannula attached. Fill the syringe with perfusion solution. Use cannulas that are 23 G for a mouse, and 18 or 16 G for a small or large rat, respectively. Slide a 1 mm length of polyethylene (PE) tubing on the end of a blunted needle (Table of Materials), to help prevent the aorta from slipping off the cannula after being tied.
4. Complete preparation of the dissection and cannulation area (Figure 1)
   1. Fill two clean weigh boats at least halfway with perfusion solution. Submerge the tip of the cannula in perfusion solution with at least one double-looped suture placed around the cannula. Hold the syringe in place with a bar clamp.
   2. Place surgical scissors, a hemostat, iris forceps, a pair of small curved scissors, and two #3 forceps for easy access.
5. Prepare the experimental system (Figure 2) by priming and circulating the modified Tyrode's solution throughout the experimental system. Ensure that the chamber is completely full and stable.
6. Turn on all data acquisition boxes, including the force transducer, the length motor, the pacing system, the temperature control system, and the data acquisition computer.
7. Copy and rename a template folder containing the required *.dap and pointer files to reference the current experiment. Open the data acquisition software.
8. Prepare the trabecula isolation tools (Vannas scissors, #5 or #55 forceps, glass probe) by submerging their tips in 10% (w/v) bovine serum albumin (BSA) in ultrapure water or perfusion solution, to coat the metal with protein (Figure 2B).
   ​NOTE: The entire experimental system must be prepared prior to dissection.

2. Gross dissection and cannulation

1. Record the identification, body weight, and other relevant information of the animal subject.
2. Optionally, inject 1,000 U/kg of heparin in sterile saline to the rodent via intraperitoneal injection at least 10 min prior to euthanasia, to minimize risks of clotting before coronary perfusion.
3. Place the animal in an induction chamber and induce general anesthesia using 3%-5% isoflurane vaporized in 100% oxygen, according to standard procedure.
   NOTE: Turn on any external scavengers, down draft tables, or fume hoods used to minimize exposure to isoflurane.
4. Once the rodent loses its righting reflex and the breathing rate has slowed:
   1. (For a rat) remove the animal from the induction chamber and place it supine on a dissection pad, with continued anesthesia through a nose cone.
   2. (For a mouse) perform cervical dislocation immediately after removing the animal from the induction chamber. The nose cone is not necessary. Turn the isoflurane vaporizer to 0%.
5. If needed, affix the upper limbs of the rodent to the dissection pad away from the chest wall using tape supported by pins, taking care not to pierce into the animal. Check for proper anesthesia depth (lack of toe-pinch response) prior to proceeding to the dissection.
6. Using surgical scissors (Mayo for rats, Metzenbaum for mice), just below the xyphoid process, cut the skin transverse across the full width of the rodent's abdomen into the peritoneal space.
7. Using surgical scissors, make two vertical cuts parasagittal (up the sides of the chest wall) on both the left and right sides of the chest wall from the transverse cut. Then, cut across the diaphragm, connecting the parasagittal cuts, and freeing the thoracic space.
8. Clamp and lift the xyphoid process using a hemostat toward the rodent's head, to move the sternum and chest wall up toward the rodent's head, exposing the thoracic space and the heart. If needed, quickly extend the parasagittal cuts to near the second vertebral space, to fully expose the heart and/or break the pericardial membrane.
9. Using curved iris forceps, carefully lift the heart to visualize the great vessels. Place the forceps between the myocardium and the spine of the rodent, clamp down on the greater vessels, and lift the heart, taking care not to clamp the atria or ventricular segments.
   1. While slightly lifting the heart, quickly place the curved iris scissors (concave up) between the curved iris forceps and the spine of the rodent, and cut the great vessels and lungs away from the heart. Quickly move the heart into a weigh boat or beaker containing fresh perfusion solution, and shake to help clear blood off of the heart. 
10. Turn the isoflurane vaporizer to 0%, if not already done. If large segments of lung, pericardial, or other tissues remain attached to the heart, carefully cut away and discard them at this time to minimize interference with the cannulation.
11. Using the curved iris forceps, move the heart into a clean weigh boat or beaker, with the prepared cannula submerged. Cannulate the aorta, secure the aorta by tightening the looped silk suture, and flush with up to 5 mL of perfusion solution.
12. Remove the heart from the cannula and place it in a silicone elastomer-coated weigh dish to prepare for trabecula isolation.

3. Isolation and equilibration of trabecula

1. Place the heart in the silicone elastomer-coated dish under a stereomicroscope and illuminate it.
2. Locate the right ventricular outflow tract. Pin the left atrium and the ventricular apex to the silicone elastomer in the dish.
3. Using long Vannas scissors, cut from the right ventricular outflow tract to the apex along the septum. Cut from the right ventricular outflow tract to the right atrium near the aorta, then cut through the right atrium (Figure 3B).
4. Using forceps, carefully pull open the right ventricular (RV) free wall from the outflow tract, being careful not to stretch the tissue.
   NOTE: Experimenters may find thin, white connective tissue strands, which can be cut without concern. Larger, pink (tissue) colored strands should be evaluated with care, as they may be trabeculae that can be isolated.
5. Pin the free wall of the right ventricle triangle to the dish, to expose the right ventricle (Figure 3C).
6. Using a glass pipette that has been melted and formed with a thin (<500 µm diameter) but not sharp end, search the exposed endocardium for free-standing trabeculae (Figure 3C).
   NOTE: A free-standing trabecula is a strip of muscle that one can fully probe underneath. Use trabeculae that have parallel sides (constant width), avoiding triangular papillary muscles. Be careful during this process and subsequent dissection, as applying strain on the trabecula can cause damage, reducing the developed force.
7. Dissect the trabecula using small Vannas scissors. Leave a ≥1 mm cubed piece of tissue at each end of the trabecula to allow for attachment. Do not stretch the trabecula where possible, and minimize contact of metal tools, as both can also cause damage to the muscle. Relocate the pins holding the heart as needed to minimize strain on the muscle near the trabeculae identified.
8. Cut ~2 inches off the end of a 7 mL transfer pipette, slowly draw the trabecula into the pipette, and transfer it into a new weigh dish containing 50% perfusion solution and 50% modified Tyrode's solution. Allow the muscle to equilibrate to the increase in extracellular calcium within the mixed solution for several minutes.
   1. Repeat steps 3.7-3.8 to dissect additional trabecula at this time, to obtain additional trabecula as backup.
9. Turn off the pump supplying superfusion and/or suction to the experimental chamber. Using the large bore transfer pipette, move the trabecula into the experimental chamber filled with Tyrode's solution.
10. Pin one >1 mm cube piece of tissue at the end of the trabecula to a hook on the force transducer, then pin the second cube to the motor.
    NOTE: Separate the motor and force transducer when mounting the first side for better access to the transducer, then move the hooks as close together as possible when mounting the second cube of tissue while the trabecula remains slack.
11. Restart superfusion and begin pacing the muscle to determine the threshold voltage. Pace at 20% above the threshold voltage for approximately 1 h to allow the trabecula to equilibrate.
12. At the end of this equilibrium period, slowly stretch the muscle using the micrometer connected to the motor until optimal developed stress generation is achieved, by observing the developed (minimum to peak) tension. Stop increasing the muscle length when the passive diastolic tension rises faster than the peak tension, indicating the optimal length has been passed.
13. Turn off the transmitted microscope illumination, and illuminate the trabecula using a gooseneck illuminator at a steep angle. Using a camera connected through the microscope optics that has previously been calibrated, capture an image of the trabecula during diastole into the experimental folder. If the trabecula is wider than the camera's field of view, take multiple images across the muscle.
14. Open the image(s) in an imaging software that reports pixel distances.
    1. Measure the pixels distance of the muscle diameter four times along its length. Measure the muscle (trabecula) length in pixels, excluding the large cube of tissue.
    2. If the muscle length is longer than the field of view, use reference points along the muscle to measure the entire length.
15. Using the template in the experimental folder, average the diameter measures, and convert the diameter and lengths from pixels to mm using a previously obtained calibration. Calculate the cross-sectional area as π*diameter²/4 in mm², and the muscle length in µm.

4. Data acquisition

1. Once the muscle is equilibrated, open the data acquisition software, select Experiments | Length Control, and enter the calibrated trabecula length (FL) and cross-sectional area (area [m²]) in the Calibration box.
2. From the template folder (Step 1.7), ensure that the freeform_file.txt points to the correct folder, and open the freeform.dap file in a text editor. Set the isotonic level (isoton) to 32,000 in the *.dap file.
3. In the Length Control box, select the Freeform tab and browse to the appropriate Freeform list file. Ensure the Data Filing path is also the correct folder to save data. Begin acquiring data from fully isometric twitches by pressing Run Experiment, before acquiring load-clamp data using a feedback control with proportional and integration gain parameters.
4. Acquire load-clamped data by defining the afterload (isoton) in the *.dap file, and iterate values of proportional gain (propgain) and integration (Ki) parameters by saving the file in the text editor. Press Run Experiment in the data acquisition software interface.
   1. Ensure that the clamp includes relengthening back to its original length (sometimes referred to as relaxation loading⁵) during this iteration to attain the maximal range of strain rates, by setting mode (flswitch) from one and increasing the threshold for ending the load-clamp (flthreshold) from zero.
5. Control the end of the load-clamp by changing the mode (flswitch) from one to zero. Repeat the acquisition while changing the end of the load-clamp from zero relengthening to complete relengthening back to the starting length.
   1. To increase the length, incrementally increase the threshold for ending the load-clamp (flthreshold) from zero until the muscle nearly relengthens back to its original length.
6. Reset the mode (flswitch = 1) and threshold (flthreshold = 0), and run one final data acquisition.
7. If desired, modify the afterload in the study by repeating steps 4.4-4.6. This acquisition may be done immediately.
8. If desired, modify the preload by stretching or shortening the muscle, or treat the muscle by adding compounds to the Tyrode's solution. If altering the preload or adding compounds, wait a minimum of 20 min to ensure that the slow-force response^9,15 has stabilized and/or for the compound to fully penetrate the muscle.
9. Once the data acquisition is completed, remove the trabecula. If needed, freeze or fix the trabecula for biochemical or histological analysis.
10. Clean the work areas and experimental system, flush all tubing with water, and turn off all components.

5. Data analysis

1. Open the data files using a quantitative analysis program by directing the program to the appropriate file path.
2. Quantify relaxation by ensuring that the data analysis program analyzes the clamped beat, and that the program correctly acquires the start of the load-clamp.
   1. Ensure that the end of the load-clamp is identified, so that the relaxation rate (1/τ) is quantified from the peak negative derivative of stress during an isometric relaxation.
   2. Use the Glantz method¹⁶ to determine this exponential time constant, or other appropriate quantification of relaxation (minimum derivative of stress, logistic time constant¹⁷, or kinematic model¹⁸).
3. Ensure that the data analysis program calculates the strain rate by taking the time derivative of the strain, where strain is calculated as the length as a function of time divided by length at optimal contraction.
4. Repeat the above steps for all traces of a given condition.
5. Plot the relationship between the relaxation rate and strain rate, limiting the maximum data to a physiological strain rate of less than 1 s^-1. Exclude data at low strain rates (Figure 4C), as the relaxation phase may not reflect the exponential decay^17,18.
6. Obtain the slope of the line between the relaxation rate and the strain rate, and record the slope as the index of MCR.
7. Repeat the above analysis for each condition interrogated.

Discussion

Mechanical control of relaxation (MCR) quantifies the dependence of the myocardial relaxation rate on the strain rate of the muscle proceeding relaxation^8,9. Strain rate, rather than afterload, is both necessary and sufficient to modify the relaxation rate⁸. As interventions to modify the calcium rate have not been proven to substantially improve cardiac relaxation, mechanical intervention may provide new insights into the mechanism and provide a new treatment for diastolic dysfunction.

The protocol to modify the myocardial strain rate described here utilizes an isotonic load-clamp^8,9. A strength of the isotonic load-clamp is the quantitative control of the afterload stress. Windkessel-like protocols could be used to further probe changes in afterload, preload, and cardiac work^2,6,7. A ramp not controlled by the load-clamp could also be used to better isolate the change in strain from strain rate. Regardless, the afterload itself does not appear to be a strong modifier of the relaxation rate⁸.

The protocol may also be adapted to approach more physiological conditions for temperature and pacing rate. The current protocol details were used to show the presence of the MCR. Conducting experiments in physiological conditions is generally recommended, depending on the experimental question. However, experiments performed at 37 °C, or at high pacing rates, can more rapidly induce rundown (damage) to the muscle. A solution with improved oxygen carrying capacity may be needed. Further, the data acquisition must be able to sample the length and force fast enough to resolve the rapid twitches and provide feedback control.

The current protocol does not describe the measurement of calcium or the measurement and control of sarcomere lengths. Calcium measurements have been addressed in other protocols¹¹, while sarcomere length measurement can be added with appropriate equipment. Sarcomere length control is not utilized in current MCR studies, because muscle length is the most correlative parameter to the clinical condition¹⁹. Further sarcomere length control (vs. muscle length control) would provide specific answers to kinetic questions, but is unlikely to add to translational knowledge due to inter-sarcomere variation and the minimum understanding of sarcomere length changes in vivo.

Three experimental considerations are highlighted here to increase the reproducibility of the data.

First, free-standing cardiac trabeculae may be difficult to find in some animals (unpublished results and communications). While twitching muscles can be found in most rats, a reasonable success rate for obtaining data from trabecula in rats is one in three. Trabecula success may be higher with Brown Norway x Lewis F1 rats, which have also been used historically²⁰ and reported to have more trabeculae (unpublished communications). For mice, success rates are likely to be lower, with less than one in 10 expected for mice from a BL/6 background; however, a higher rate is expected for mice from a FVBN background (unpublished communications and observations).

Second, damage to muscles can reduce output. If the developed forces are less than 10 mN mm^-2 at 25 °C and 0.5 Hz pacing, investigators may need to perform troubleshooting to assess if inadvertent stretching or contact between metal forceps and muscle is occurring, if solutions are not properly prepared, or if pacing or experimental equipment is functioning properly. Other protocols utilizing intact trabecula have suggested using Luer-lock syringes as transfer vessels¹¹. While this is possible, especially if the user controls a very slow flow rate or smaller muscle segment, the current protocol utilizes a much larger bore transfer pipette to minimize possible damage. Another step where ischemic damage may occur is during dissection. The aorta should be cannulated and flushed with perfusion solution within 3 min of the first abdominal cut (rat) or cervical dislocation (mouse), similar to limits listed in cardiomyocyte isolation protocols^21,22. This minimizes the time when the cardiac tissue is not exposed to the cardioplegia-like perfusion solution. Further, dissections lasting more than 30 min generally do not produce twitching trabecula. Thus, operators should practice rapid but careful dissection to minimize damage. A cross-sectional area above 0.2 mm² (2 x 10^-7 m²) may suffer from core ischemia²⁰.

Third, the way in which muscles are attached to the motor and force transducer should be considered. This protocol currently focuses on hooks and free-standing trabeculae. The sometimes rapid strain rate of the stretch prior to relaxation can cause a muscle to slide if not affixed properly, which is why the current protocol does not use "baskets" to hold the trabecula^23,24. Alternate mounting methods (adhesives, clips, etc^25,26) can also be considered and validated. The protocol described here utilizes trabeculae and not papillary muscles. The chordae of the papillary muscle induce a series elasticity that can inhibit changes to the MCR⁹. However, the exact placement of the attachments in the muscle is unlikely to impact the measures, because the trabecula length (and diameter) vary substantially.

A limitation of piercing the muscle ends with hooks is that the mounting point itself can also be damaged. Possible tearing of the affixed muscle tissue with frequent contractions (depending on their strength) may change the length or series elasticity. This rate of tearing is difficult to control. Similarly, damage to the tissue and the hook can be exacerbated during stretching, also potentially causing problems. Visual inspection, and the developed force values remaining >80% of the equilibrated isometric force, should be used to assess whether the preparation is damaged and should be excluded.

Another limitation or consideration affects what experimental questions can be answered by the method. For example, consider the use of 2,3-butanedione monoxime (BDM) in the perfusion solution. BDM is a phosphatase, which may alter the function of the muscle. In addition, the long period of unloading and lack of pacing mean that the latent phosphorylation state has likely changed. Thus, caution should be used if trying to directly assess the muscle contractility of an animal (vs. differences between genotypes or treatments), as the contractile state has likely changed. However, the impact of phosphorylation may be assessed pharmacologically by adding an agonist or antagonist of the pathway.

In summary, MCR provides insight into how relaxation is regulated by muscle movement (strain rate). MCR may help to provide better insight into the diagnosis and monitoring of diastolic disease, along with targets for pharmacological intervention, such as modifying myosin kinetics. The protocol and advice outlined here lay out knowledge developed over several years of trials, and should be applicable to other systems and models of cardiac disease.

Materials

Name	Company	Catalog Number	Comments
18 or 16 gauge blunted needle/canula			for cannulation of rat aorta, use 1mm of PE160 or PE205 tubing as stop
2,3-Butanedione Monoxime	Sigma-Aldrich	B0753-25G
23 gauge blunted needle/canula			for cannulation of mouse aorta, use 1mm of PE50 tubing as stop
5 mL syringe	BD Luer-Lock	309646
95% Oxygen/5% CO2	AirGas	Z02OX9522000043
Anethesia system	EZ Systems	EZ-SA800	Can use any appropriate anethesia method/system
Bovine Serum Albumin	Fisher BioReagents	BP-1600	to coat tips of fine forcepts, scissors
Calcium Chloride Dihydrate	Fisher Chemical	C79-500
Containers/dissection dishes	FisherBrand	08-732-113	Weigh dishes for creating dissection plates
Crile Hemostat	Fine Science Tools	13005-14	for mouse gross dissection
D-(+)-Glucose	Sigma-Aldrich	G8270-1KG
Data acquisition software	SLControl
Data acquisition system	MicrostarLabs	DAP5216a	Can use any DAQ.  This is a PCI based data acqusition for use with SLControl; must have a PC with a PCI slot
Data analysis software	Mathworks	Matlab	Custom Script
Dumont #3 Forceps	Fine Science Tools	11231-30	2x for cannulation of aorta
Dumont #5 Forceps	Fine Science Tools	11254-20	2x for trabecula isolation
Experimental system	Aurora Scientific	801C	Can use any appropriate experimental chamber with force and length control
Fine Scissors, curved	Fine Science Tools	14061-09	for removal of heart
Gooseneck Piggyback Illuminator	AmScope	LED-6WA
HEPES	Sigma-Aldrich	H3375-250G
Imaging software	IrfanView
Iris Forceps	World Precision Instruments	15915	for removal of heart
Isoflurane	VetOne	502017
Magnesium Chloride Hexahydrate	Sigma-Aldrich	M2670-100G
Magnesium Sulfate	Sigma-Aldrich	M7506-500G
Mayo Scissors	Fine Science Tools	14110-15	for rat gross dissection
Metzenbaum Scissors	Fine Science Tools	14116-14	for mouse gross dissection
Microscope connected camera	Flir	BFS-U3-27S5M-C	Includes acquisition software
Microscope/digital imaging system	Olympus	IX-73	Can use any appropriate microscope.  Needed to measure muscle length, cross sectional area
Mounting Pin/Needle	BD PrecisionGlide	305136	For holding heart to dish.  27 G x 1-1/4
Mounting Pin/Needle	Fine Science Tools	26000-40	For holding heart to dish. 0.4mm diameter insect pin (Alt to 27G needle)
Oxygen (O2)	AirGas	OX USP300
Peristaltic Pump	Rainin	Rabbit	Can be any means to create flow in experimental chamber
pH and Oxygen sensor	Mettler Toledo	SevenGo pH and DO
Potassium Bicarbonate	Sigma-Aldrich	237205-100G
Potassium Chloride	Fisher Chemical	P217-500
Potassium Phosphate Monobasic	Sigma-Aldrich	795488-500G
Rochester-pean Hemostat	World Precision Instruments	501708	for rat gross dissection
Silk Suture, Size: 4/0	Fine Science Tools	18020-40	cut to ~1.5 inch pieces, soaked in water
Sodium Bicarbonate	Sigma-Aldrich	S6297-250G
Sodium Chloride	Sigma-Aldrich	S9888-1KG
Sodium Hydroxide	Sigma-Aldrich	S8045-500G
Sodium Phosphate Dibasic	Sigma-Aldrich	S7907-100G
Stereomicroscope	AmScope	SM-1TX
Student Vannas Spring Scissors	Fine Science Tools	91500-09	for opening of the RV
SYLGARD 184 Silicone Elastomer Base	Dow Corning	3097358-1004	For creating dissection plates
Syringe Holder	Harbor Frieght	Helping Hands 60501	Can be used as alternate for ring stand
Taurine	Sigma-Aldrich	T0625-1KG
Transfer Pipette	FisherBrand	13-711-7M	cut ~1" from tip to widen bore
Vannas Spring Scissors	Fine Science Tools	15000-00	for trabecula isolation