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Fast Inspection of Quality of Indigo Naturalis by Multiple Light Scattering
In This Article
Summary
Here, we present a quality evaluation method based on a multiple light scattering technique to evaluate the quality of Indigo Naturalis.
Abstract
Quality control of Chinese herbal medicine is a crucial component of Chinese herbal medicine research and development. Faced with the challenges of modernization and internationalization of Chinese herbal medicine, it is urgent to establish thorough and effective procedures for quality identification of Chinese herbal medicine, and there is an urgent need for new analytical and testing techniques that are efficient, accurate, and environmentally friendly.
Multiple light scattering is a cutting-edge and analytical method that can accurately and rapidly assess the quality of Chinese herbal medicine without altering the nature or state of the sample or using organic reagents. Indigo Naturalis is considered a good remedy for pediatric hyperthermia, psoriasis, leukemia, and ulcerative colitis. In this study, the process of addition of Indigo Naturalis powder in water was recorded precisely using a multiple light scattering instrument.
The qualitative and quantitative measurements of the instrument can be used to accurately capture the overall trajectory and sinking behavior of Indigo Naturalis powder into water and to establish a rapid evaluation method for the quality of Indigo Naturalis with the transmission and backscattering spectrograms of the sample as qualitative indicators and stability index as a quantitative indicator. The analytical technique based on multiple light scattering provides a fast, accurate, green, and environmentally friendly method for the quality evaluation of Indigo Naturalis and supports the development and transformation of high-quality Indigo Naturalis.
Introduction
In traditional Chinese medicine, during the course of disease treatment, the clinical effectiveness of medications and the safety of the course of treatment are directly impacted by the quality of Chinese herbal medicine. Utilizing cutting-edge identification technology, one can assess the efficacy of Chinese herbal medicine and guarantee user safety. The Chinese herbal medicine water test method refers to immersing the herbs in water or solvent, then quickly and precisely determining the medicine's authenticity by observing the changes in color, size, and shape1.
It was originally a good choice for the identific....
Protocol
1. Preparation of test sample
- Set up four different batches of Indigo Naturalis herbal powder for the test. Pass each sample through the seventh sieve and ninth sieve in turn and collect the sample between the seventh and ninth sieve5.
NOTE: The average aperture size of the seventh sieve is 125 µm ± 5.8 µm. The average aperture size of the ninth sieve is 75 µm ± 4.1 µm. - Accurately weigh 0.2 g of the sample (Indigo Naturali.......
Representative Results
Figure 2A-D correspond to S1, S2, S3, and S4 of Indigo Naturalis, respectively. A is of high-quality Indigo Naturalis, showing the same light transmittance at any height within 0-10 min, which is very stable. B is the common Indigo Naturalis, and its light transmittance fluctuates slightly with the change of time and is generally stable. C and D are fake and inferior products. There may be two conditions in the transmission spectrograms of fake Indigo Natura.......
Discussion
According to traditional Chinese medicine, Indigo Naturalis has the power to clear heat and detoxification, cool blood, eliminate spots, purge fire, and arrest convulsion. Based on randomized double-blind controlled clinical trials11,12,13, Indigo Naturalis is effective in the treatment of psoriasis, ulcerative colitis, and acute promyelocytic leukemia in addition to its traditional treatment of cough and phlegm, hemorrhagic sym.......
Acknowledgements
The work wishes to acknowledge support from the National Natural Science Foundation of China (No. 82173976), National Key Research and Development Program (No. 2018YFC1707205), and State Key Laboratory of Innovative Drugs and High Energy Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine (No. GZSYS202003).
....Materials
| Name | Company | Catalog Number | Comments |
| Analytical balance (1/10,000) | Sartorious, Germany | BSA224S | www.sartorius.com.cn |
| Funnel | Chengdu Kelong Chemical Co. LTD | Diameter 5 cm | www.cdkelongchem.com |
| Indigo Naturalis S1 | Xianyou, Fujian | 20210501 | |
| Indigo Naturalis S2 | Yaan, Sichuan | 20201102 | |
| Indigo Naturalis S3 | Xianyou, Fujian | 20161012 | |
| Indigo Naturalis S4 | Xianyou, Fujian | 20180305 | |
| Iron ring | Chengdu Kelong Chemical Co. LTD | / | www.cdkelongchem.com |
| Iron stand | Chengdu Kelong Chemical Co. LTD | / | www.cdkelongchem.com |
| Mili-Q ultra-pure water meter | Milipore, USA | Mili-Q | www.merckmillipore.com |
| Ninth sieve | Chengdu Kelong Chemical Co. LTD | Average aperture size 75 µm | www.cdkelongchem.com |
| Sample bottle | French Formulaction Company | Bottom diameter 2.6 cm, height 6 cm | www.formulaction.com |
| Seventh sieve | Chengdu Kelong Chemical Co. LTD | Average aperture size 125 µm | www.cdkelongchem.com |
| Turbisoft Lab multiple light scattering instrument | French Formulaction Company | Turbisoft Lab 2.3.1.125 Fanalyser 1.3.5 | www.formulaction.com |
| Weighing paper | Chengdu Kelong Chemical Co. LTD | / | www.cdkelongchem.com |
References
- Li, G. M. The application of water test in the identification of traditional Chinese medicine. Chinese Medicine Modern Distance Education of China. 19 (23), 153-155 (2021).
- Ye, B.
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