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Shanghai Jiaotong University

13 ARTICLES PUBLISHED IN JoVE

Bioengineering

Preparation and Characterization of Lipophilic Doxorubicin Pro-drug Micelles

Feng Li¹, Candace Snow-Davis¹, Chengan Du¹, Mikhail L. Bondarev¹, Marilyn D. Saulsbury¹, Simone O. Heyliger¹

A protocol for the preparation and characterization of lipophilic doxorubicin pro-drug loaded 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) micelles is described.

JoVE Journal

Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling

Hong Yang¹, Shan Yu Fung², Aihua Bao¹, Qiang Li¹, Stuart E. Turvey²

¹Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, ²Department of Pediatrics, BC Children's Hospital and University of British Columbia

Toll-like receptor (TLR) signaling plays an important role in the pathophysiology of many human inflammatory diseases, and regulating TLR responses by bioactive nanoparticles is anticipated to be beneficial in many inflammatory conditions. THP-1 cell-based reporter cells provide a versatile and robust screening platform for identifying novel inhibitors of TLR signaling.

Medicine

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

Zhongwei Sun^1,2, Feng Li³, Xin Zhou³, Wen Wang^1,2

¹Cellular Biomedicine Group, Shanghai, ²Cellular Biomedicine Group, Cupertino, ³Department of Respiratory Medicine, Shanghai General Hospital, Shanghai Jiaotong University

This study describes the successful generation of a new chronic obstructive pulmonary disease (COPD) animal model by repeatedly exposing mice to high concentrations of ozone.

Neuroscience

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method

Weiyue Zhang^*1, Wei Zhang^*1, Ning Dai^*1, Chenxia Han², Fengzhi Wu¹, Xu Wang¹, Libo Tan¹, Jie Li¹, Feng Li¹, Qingjia Ren³

¹School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, ²Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, ³Institute of Tibetan Medicine, Tibetan Traditional Medical College

Here, we present a protocol to introduce a rat model of central fatigue using the modified multiple platform method (MMPM).

JoVE Journal

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Dan Li^*1, Feng Li^*1, Pavithran Guttipatti¹, Yuanquan Song^1,2

¹Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine, University of Pennsylvania

Here, we present a protocol using the Drosophila sensory neuron - dendritic arborization (da) neuron injury model, which combines in vivo live imaging, two-photon laser axotomy/dendriotomy, and the powerful fly genetic toolbox, as a platform for screening potential promoters and inhibitors of neuroregeneration.

Bioengineering

Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles

Hisato Konoeda¹, Hong Yang², Chengliang Yang¹, Annette Gower¹, Chun Xu¹, Wei Zhang¹, Mingyao Liu^1,3

¹Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, ²Respiratory Medicine Research Laboratory, Institute of Translation Medicine, Shanghai General Hospital, Shanghai Jiaotong University, ³Institute of Medical Science, Faculty of Medicine, University of Toronto

We have previously used a gold nanoparticle peptide hybrid to intravenously deliver a synthetic peptide, protein kinase C-delta inhibitor, which reduced ischemia-reperfusion-induced acute lung injury. Here we show the detailed protocol of the drug formulation. Other intracellular peptides can be formulated similarly.

Establishment of Liver Cancer Patient-derived Xenograft Models

Chuan Xing Wu^1,2, Jia Yi Chen¹, Kun Dong Zhang¹, Dong Wang Yan¹, Xiao Qi Wang², Zheng Jun Qiu¹

¹Department of General Surgery, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, ²Department of Surgery, University of Hong Kong

Here, we present a protocol to establish liver cancer patient-derived xenograft models for the preclinical study of novel anticancer drugs.

Genetics

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Xuan Su^*1,2, Yan Shen^*1, Yue Jin^*1,2, Meng Jiang², Neal Weintraub¹, Yaoliang Tang¹

¹Vascular Biology Center, Medical College of Georgia, Augusta University, ²Renji Hospital, School of Medicine, Shanghai Jiaotong University

Here, we present a protocol to transiently improve cardiac function in Duchenne muscular dystrophy mice by transplanting exosomes derived from normal myogenic progenitor cells.

Developmental Biology

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Yue Jin¹, Yan Shen¹, Xuan Su¹, Neal Weintraub¹, Yaoliang Tang¹

¹Medical College of Georgia, Augusta University

Here, we present a Cas9-based exon23 deletion protocol to restore dystrophin expression in iPSC from Dmd^mdx mouse-derived skin fibroblasts and directly differentiate iPSCs into myogenic progenitor cells (MPC) using the Tet-on MyoD activation system.

Developmental Biology

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Wei Zhang^*1, Xinjuan Lei^*1, Xin Zhou^*2,3, Boling He¹, Liqin Xiao¹, Huimin Yue¹, Shulin Wang¹, Yuting Sun¹, Yajun Wu¹, Liyang Wang^1,4, George Ghartey-Kwansah¹, Odell D. Jones⁵, Joseph L. Bryant⁶, MengMeng Xu⁷, Jianjie Ma³, Xuehon Xu¹

¹National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, ²Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, ³Ohio State University College of Medicine, ⁴Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, ⁵University of Pennsylvania ULAR, ⁶University of Maryland School of Medicine, ⁷Columbia University Medical Center

Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.

Medicine

Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model

Sijuan Sun^*1, Hongbin Zhu^*2, Shoubao Wang³, Xiuxia Xu⁴, Lincai Ye^2,5,6

¹Department of Pediatric Intensive Care Unit, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, ²Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiaotong University, ³Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, ⁴Department of Radiology, Huangpu Branch, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, ⁵Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, ⁶Shanghai Institute for Pediatric Congenital Heart Disease, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University

This protocol presents the establishment and confirmation of a postnatal right ventricular volume overload (VO) model in mice with abdominal arteriovenous fistula (AVF), which can be applied to investigate how VO contributes to postnatal heart development.

Biology

Establishment of Facial Nerve Injury Rat Model for Idiopathic Facial Paralysis Research

Xie Yandong^1,2, Ying Tingting^1,2, Liu Liang³, Liu Mingxi³, Wang Ran³, Li Shiting^1,2, Xin Zhang^1,2

¹Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, ²The Cranial Nerve Disease Center, Shanghai Jiaotong University, ³Department of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University

The present protocol establishes a facial nerve injury rat model using microscopy to investigate the diagnostic and therapeutic mechanisms of idiopathic facial paralysis.

Neuroscience

Preliminary Validation of Stereotaxic Injection Coordinates via Cryosectioning

Xiaoxin Zhou^*1,2,3, Wanbing Dai^*1,2, Jie Zhou^*1,2, Yizhe Zhang^1,2, Xiao Zhang^1,2, Sihan Chen^1,2, Xiaoyu Sun^1,2

¹Department of Anesthesiology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiaotong University

The present protocol describes a practical strategy to expedite the verification step of stereotaxic injection coordinates before conducting viral tracing using dyes and frozen sections.