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The Second Clinical College of Guangzhou University of Chinese Medicine

7 ARTICLES PUBLISHED IN JoVE

Behavior

Bouncing Ball with a Uniformly Varying Velocity in a Metronome Synchronization Task

Yingyu Huang^*1, Li Gu^*1, Junkai Yang^*1, Xiang Wu¹

¹Department of Psychology, Sun Yat-Sen University

The purpose of this protocol is to introduce the application of a bouncing ball with a uniformly varying velocity in a metronome synchronization task.

Medicine

A Detailed Protocol for Physiological Parameters Acquisition and Analysis in Neurosurgical Critical Patients

Xiang Wu^1,2, Guoyi Gao^1,2, Junfeng Feng^1,2, Qing Mao^1,2, Jiyao Jiang^1,2

¹Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, ²Shanghai Institute of Head Trauma

Recently, we have developed a portable multimodality monitoring system for the monitoring of various physiological parameters in neurosurgical critical patients. Detailed protocols on how to use this multimodality monitoring system are presented here.

Medicine

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Guihao Chen¹, Yuejin Yang¹, Chuansheng Xu², Shuo Gao²

¹State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, ²State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

JoVE Journal

A 100 KW Class Applied-field Magnetoplasmadynamic Thruster

Baojun Wang¹, Haibin Tang², Yibai Wang¹, Chao Lu¹, Cheng Zhou³, Yangyang Dong¹, Ge Wang³, Yuntian Cong³, Daniel Luu⁴, Jinbin Cao²

¹Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Astronautics, Beihang University, ²Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Space and Environment, Beihang University, ³Beijng Institute of Control Engineering, ⁴School of Aerospace Engineering and Geodesy, University of Stuttgart

The goal of this protocol is to introduce the design of a 100 kW class applied-field magnetoplasmadynamic thruster and relevant experimental methods.

Neuroscience

In Vivo Methods to Assess Retinal Ganglion Cell and Optic Nerve Function and Structure in Large Animals

Qian Ye^*1, Zhonghao Yu^*1, Tian Xia^*1, Shengjian Lu¹, Jiaying Sun¹, Mengyun Li¹, Yu Xia¹, Si Zhang¹, Wencan Wu¹, Yikui Zhang¹

¹The Eye Hospital, School of Ophthalmology & Optometry, Wenzhou Medical University

Here we demostrate several in vivo tests (flash visual evoked potential, pattern electroretinogram and optic coherence tomography) in goat and rhesus macaque to understand the structure and function of the optic nerve and its neurons.

Medicine

Laparoscopic Radical Antegrade Modular Pancreatosplenectomy via Dorsal-Caudal Artery Approach for Pancreatic Neck-Body Cancer

Zhantao Shen^1,2, Guihao Chen^1,2, Chunbao Zhu², Xiang Wu^1,2, Yifeng Liu^1,2, Zhangyuanzhu Liu^1,2, Zhijian Tan^1,2, Xiaosheng Zhong^1,2

¹The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, ²The Second Clinical College of Guangzhou University of Chinese Medicine

With advancements in laparoscopic techniques, laparoscopic radical antegrade modular pancreatosplenectomy (L-RAMPS) has been widely recognized. However, owing to several technical difficulties in this procedure, the artery-first approach in L-RAMPS still remains uncommon. Here, we developed the dorsal-caudal artery approach for L-RAMPS, which might be safe and beneficial for pancreatic neck tumors.

Genetics

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

Yu Xia^*1,2, Jinlin Hu^*3, Xiang Li⁴, Shuang Zheng⁴, Ge Wang^1,2, Songtao Tan^1,2, Zengxiao Zou^1,2, Qiong Ling^2,5, Fenghua Yang⁴, Xiaoping Fan^1,2

¹Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, ²The Second Clinical College of Guangzhou University of Chinese Medicine, ³Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, ⁴Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, ⁵Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.