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Chongqing 13th People's Hospital

6 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Yiding Qi^*1, Xiang Miao^*2, Lian Xu³, Mengxia Fu³, Shi Peng⁴, Kailei Shi⁵, Jun Li⁴, Maoqing Ye⁵, Ruogu Li¹

¹Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, ²Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, ³Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, ⁴Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, ⁵Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University

We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre^+/-;Rosa26^RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.

Bioengineering

Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy

Chien Poon¹, Ben Rinehart¹, Jun Li^1,2, Ulas Sunar¹

¹Department of Biomedical, Industrial and Human Factors Engineering, Wright State University, ²South China Academy of Advanced Optoelectronics, South China Normal University

This protocol demonstrates how to measure resting state functional connectivity in the human prefrontal cortex using a custom-made diffuse correlation spectroscopy instrument. The report also discuss practical aspects of the experiment as well as detailed steps for analyzing the data.

Medicine

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

Hang You^1,2, Xin Mao², Chenyang Wang², Gang Huang³, Marcus Groettrup^4,5, Jun Li²

¹School of Medicine, Chongqing University, ²Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, ³Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, ⁴Division of Immunology, Department of Biology, University of Konstanz, ⁵Biotechnology Institute Thurgau, University of Konstanz

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

Medicine

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Xin Mao^*1, Peng Xian^*1, Hang You^1,2, Gang Huang³, Jun Li¹

¹Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, ²School of Medicine, Chongqing University, ³Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University

In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

Medicine

Microscopic Replantation of Penile Glans Amputation Due to Circumcision

Da-Chun Jin^*1, Bo Zhou^*1, Jun Li², Cong-Cong Bao¹, Yong Luo¹, Yong Zhang¹, Peng Wang¹, Gang Bi¹, Yan-Feng Li¹

¹Department of Urology, Daping Hospital, Army Medical University, ²Department of Urology, Chongqing 13th People's Hospital

The present protocol describes the emergency management of microscopic replantation of penile glans amputation due to circumcision.

Bioengineering

Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall

Mieradilijiang Abudupataer^*1, Xiujie Yin^*1, Bitao Xiang^*1, Nan Chen¹, Shiqiang Yan², Shichao Zhu¹, Yang Ming¹, Gang Liu¹, Xiaonan Zhou¹, Hao Lai¹, Chunsheng Wang¹, Kai Zhu¹, Jun Li¹

¹Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, ²Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University

Here, we developed a human aorta smooth muscle cell organ-on-a-chip model to replicate the in vivo biomechanical strain of smooth muscle cells in the human aortic wall.