Monocytes Anti-human CD14 Magnetic Particle Isolation
4:08
Monocyte Interleukin (IL)-4 and Granulocyte Macrophage–Colony Stimulating Factor (GM-CSF) Stimulation
5:10
Results: Representative Human Monocyte–Dendritic Cell Generation from Whole Blood
6:19
Conclusion
Transkript
The overall goal of this CD14 magnetic beads separation technique is to isolate monocytes from the blood for their further differentiation into dendritic cells or macrophages in vitro. This method can help answer key questions in the field of immu
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Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.