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Generation of Human Monocyte-derived Dendritic Cells from Whole Blood (Video) | JoVE

DOI :

10.3791/54968-v

December 24th, 2016

December 24th, 2016

19,405 Views

1Division of Hygiene and Medical Microbiology, Medical University of Innsbruck

Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.

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