Visualization and Targeting of GFP+ Retinal Ganglion Cells
4:30
Cell Isolation
5:50
RNA Purification
8:38
Results: Evaluation of Cell Isolation and Sequencing Preparation
10:28
Conclusion
Transkript
The overall goal of this procedure is to isolate fluorescently labeled single cells from live whole mount retinas. This method can help answer key questions in neuroscience. Such as how many subtypes of a particular neuronal class exist and what a
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Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.