Crosslink of Protein-RNA Interactions in Live Cells
3:26
Isolation of Nuclei
4:27
Lysis of Nuclei and Trypsin Digestion
5:51
Desalting of Peptides
7:39
Removal of Crosslinked RNA
8:44
Results: RNA-binding Protein and RNA-binding Region Identification
10:04
Conclusion
Transkript
The overall goal of this procedure is to identify RNA binding proteins in live cells and map their RNA binding regions using UV mediated photo cross-linking followed by mass spectrometry. This method can help answer key questions in the field of e
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We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.