The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
Applying RNAi to Nematodes Using a Feeding Protocol
7:57
Analyzing Phenotypes Using Fluorescence Dissecting Microscopy
8:41
Analyzing Phenotypes Using Confocal Microscopy
10:14
Results: Immunohistochemistry, RNAi and Microscopic Analysis of Intestinal Apical membrane and Lumen Morphogenesis
11:45
Conclusion
Transkript
The overall goal of this combination of standard methods is to demonstrate how the C.elegans intestine can be used for the in vivo analysis of polarized membrane biogenesis and lumen morphogenesis at the single-cell and sub-cellular level. This me
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The transparent C. elegans intestine can serve as an "in vivo tissue chamber" for studying apicobasal membrane and lumen biogenesis at the single-cell and subcellular level during multicellular tubulogenesis. This protocol describes how to combine standard labeling, loss-of-function genetic/RNAi and microscopic approaches to dissect these processes on a molecular level.