Tartrate Resistant Acid Phosphatase Staining and Activity Quantification of Culture Medium
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Results: Deletion of Raptor Impaired Osteoclast Formation
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Conclusion
Transkript
The overall goal of this protocol is to isolate and culture osteoclasts in vitro from mouse bone marrow and to study the role of the mammalian target of rapamycin complex one in osteoclast formation. This method can help answer key questions in th
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This manuscript describes a protocol to isolate and culture osteoclasts in vitro from mouse bone marrow, and to study the role of the mammalian/mechanistic target of rapamycin complex 1 in osteoclast formation.