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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

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14:25 min

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December 12th, 2017

DOI :

10.3791/57092-v

December 12th, 2017

•
17,375 Views
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, 2Department of Microbiology, Monash University, 3Department of Biochemistry and Molecular Biology, Monash University

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Keywords Chemoreceptor
Ligand Binding Domain
Refolding
Purification
Inclusion Bodies
E Coli
Protein Expression
IPTG Induction
Cell Lysis
Homogenization
Centrifugation
SDS PAGE
Buffer Exchange
Solubilization
Protein Purification
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