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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

17.5K Views

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14:25 min

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December 12th, 2017

DOI :

10.3791/57092-v

December 12th, 2017

•
17,478 Views
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, 2Department of Microbiology, Monash University, 3Department of Biochemistry and Molecular Biology, Monash University

Trascrizione

Esplora Altri Video

Chemoreceptor
Ligand Binding Domain
Refolding
Purification
Inclusion Bodies
E Coli
Protein Expression
IPTG Induction
Cell Lysis
Homogenization
Centrifugation
SDS PAGE
Buffer Exchange
Solubilization
Protein Purification
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