Transfection of Cells to Introduce the Caspase Bimolecular Fluorescence Complementation (BiFC) Components
2:42
Three-dimensional Imaging of Caspase BiFC
5:00
Time-lapse Imaging of Caspase BiFC
6:19
Results: Visualization of Caspase Activation Pathways Using Caspase Bimolecular Fluorescence Complementation
7:37
Conclusion
Transkript
The overall goal of this methodology is to visualize one of the first steps in the activation of an initiator caspases. This step is called induced proximity and occurs when caspase molecules come together to form active dimers during apoptotic ce
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This protocol describes caspase Bimolecular Fluorescence Complementation (BiFC); an imaging-based method that can be used to visualize induced proximity of initiator caspases, which is the first step in their activation.