Establishing Human-derived 3D Gastric Fundic Organoids
5:23
Transfer of 3D m/hFGOs to 2D Gastric Epithelial Cell Monolayers
7:44
Scratch Wound Assay Using 2D Gastric Epithelial Cell Monolayers
9:12
Results: Use of Gastric Epithelial Monolayer as a Scratch-wound Assay for Gastric Regeneration
10:53
Conclusion
Transkript
The overall goal of this culture system is to generate a primary mouse or human-derived gastric monolayer for the study of regeneration in a wound healing assay. This method can help answer key questions in the gastric field such as the mechanism
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
Here we describe a protocol for establishing and culturing human- and mouse-derived 3-dimensional (3D) gastric organoids, and the method for the transfer of 3D organoids to a 2-dimensional monolayer. The use of the gastric epithelial monolayer as a novel scratch-wound assay for regeneration studies is also described.