Integration Strand Transfer Assay, Fraction Selection, and Storage
8:29
Results: Isolation and Evaluation of PFV Intasomes
9:45
Conclusion
Transkript
The overall goal of this procedure is to assemble and purify prototype foamy virus intasomes which may be then be used for biochemical, structural and kinetic studies. This method can help answer key questions in the retrovirology field such as th
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Recombinant retroviral integrase and DNA oligomers mimicking viral DNA ends can form an enzymatically active complex known as an intasome. Intasomes may be used for biochemical, structural, and kinetic studies. This protocol details how to assemble and purify prototype foamy virus intasomes.