Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
3:52
PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
5:00
In Vitro RAW264.7 Macrophages Transfection
6:55
Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
8:51
Conclusion
Transkript
This measure can help answer key questions. In the field of humanology. That answer in the function of macrophages under physiological end to pathological conditions.
The main advantage of this technique is that polyethyleneimine coated can
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We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.