Our laboratory at Johns Hopkins has been performing small animal kidney and liver transplantation with over two decades of experience. This specific protocol outlines critical steps that are necessary for successfully performing orthotopic kidney
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The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.