Primary Antibody Incubation and PLA Probe (Secondary Antibody) Incubation
5:21
Ligation
6:09
Amplification and Final Washes
6:45
Coverslip Mounting
7:15
Results: Germlines Following Co-immunostaining or PLA
8:10
Conclusion
Transkript
Proximity ligation assay allows users to detect protein-protein interactions in situ, revealing spatial and temporal patterns of the interactions. This technique can help answer important questions about regulation of C.elegans development. This t
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This protocol demonstrates use of the proximity ligation assay to probe for protein-protein interactions in situ in the C. elegans germline.