Results: Green Fluorescent Protein (GFP) Expression Using the Autolysate
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Conclusion
Transkript
Using this protocol, high-quality bacterial cell lysates can be produced using only broadly available common laboratory equipment, making cell-free gene expression easily accessible to most researchers. Combining program cellular autolysis with a
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This protocol describes a rapid and simple method to produce bacterial lysate for cell-free gene expression, using an engineered strain of Escherichia coli and requiring only standard laboratory equipment.