Preparation of Feeding Chamber and Artificial Diet
2:00
Collection of Small Brown Planthopper (SBPH) Saliva
2:56
Concentration of the Collected Saliva
3:58
Results: Artificial Feeding and Saliva Collection from Laodelphax striatellus
5:21
Conclusion
Transkript
Artificial feeding and saliva collection provide a direct method for detecting effectors in insect saliva. However, this has not be described in detail before. This protocol is effective for saliva collection from saprophytic insects.
Additi
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The present protocol describes a method to collect sufficient saliva from piercing-sucking insects using an artificial medium. This is a convenient method for collecting insect saliva and studying salivary function on insect feeding behavior and vector-borne virus transmission.