Generation of Surface-Immobilized Microtubule Bundles
4:57
Image Microtubule Dynamics
5:53
Results: TIRF Microscopy of Crosslinked and Single Microtubules
6:35
Conclusion
Transkript
Cellular microtubule arrays contains subpopulations of microtubules with distinct dynamic properties as required for function. This protocol addresses how the collective activity of regulators bestows proximal microtubule subpopulations with disti
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Here, a TIRF microscopy-based in vitro reconstitution assay is presented to simultaneously quantify and compare the dynamics of two microtubule populations. A method is described to simultaneously view the collective activity of multiple microtubule-associated proteins on crosslinked microtubule bundles and single microtubules.