Results: Tyrode s Buffer Sensitivity to L. major Proteins Compared to M199 and MEK Pathway Activation in L. major
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Conclusion
Transkript
The mechanism of resistance to pore-forming toxins is poorly understood. Our protocol enables function and mechanism to pore-forming toxins using Leishmania major, a genetically tractable and physiologically relevant system. The main advantage of
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Presented here is a protocol using Leishmania major promastigotes to determine the binding, cytotoxicity, and signaling induced by pore-forming toxins. A proof-of-concept with streptolysin O is provided. Other toxins can also be used to leverage the genetic mutants available in L. major to define new mechanisms of toxin resistance.