This protocol demonstrates a completely new way to analyze low affinity protein complexes. It allows researchers to detect and characterize certain protein interactions even when they are weak or transient, which has generally been very difficult or even impossible before. Protein interactions are crucial for cell function, but detecting and measuring them can be challenging.
Mass photometry is a great tool for quantifying protein complex formation, but it has limitations with dilute samples. We're solving this problem with MassFluidix. The research gap we are addressing is how scientists can reliably characterize low affinity interactions between proteins in solution.
The advantages of our approach are that it allows you to look for weak protein interactions in solution without the need for labels or immobilization. So researchers can observe the proteins of interest and how they interact without worrying that they're disrupting the system they're looking at.
