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Abstract

Medicine

DNA Extraction and Comparison Between Old and Fresh Necrophilic Fly Samples

Published: May 3rd, 2024

DOI:

10.3791/66737

1Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (tropical forensic medicine), 2Hainan Vocational College of Political Science and Law, Hainan Gongping Forensic Cente, 3Department of Forensic Medicine, Hebei Medical University

* These authors contributed equally

ERRATUM NOTICE

Important: There has been an erratum issued for this article. Read more …

Abstract

A total of five samples of Chrysomya megacephala samples - three fresh samples, one sample stored in alcohol for 2 years, and one sample stored in dry sealed storage for 2 years protected from light only - were selected to investigate whether a blood DNA extraction kit could extract DNA from necrophilous flies and to determine whether alcohol could prolong the preservation of necrophilous flies' DNA. First, the blood DNA extraction kit was used to extract DNA from their thorax tissues. Then, the DNA purity and concentration were examined using a microplate reader and a fluorometer. Finally, PCR amplification and electrophoresis of the extracted DNA were done with necrophilic fly-specific primers located in the mitochondrial CO I gene sequence. The results showed that the DNA purity of all samples was greater than 2.0. The DNA concentration was observed to be of the following order: fresh samples > alcohol-preserved old samples > untreated, old samples. All samples had specific electrophoretic bands after PCR amplification. In conclusion, a blood DNA extraction kit can be used to extract DNA from necrophilic flies successfully, and the DNA concentration of fresh fly samples is greater than that of old fly samples. The flies can be stored in alcohol for a long time.

Erratum

Erratum: DNA Extraction and Comparison Between Old and Fresh Necrophilic Fly Samples

This corrects the article 10.3791/66737

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