<meta charset="utf8"></head><body>Epilepsi Araştırmaları için Esnek Çok Bölgeli Nöral Kayıt Cihazı

<ol>
	<li>Devinsky, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epilepsy.">Epilepsy.</a> Nat Rev Dis Primers. 4, 18024 (2018).</li><li>Piper, R. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Towards+network-guided+neuromodulation+for+epilepsy.">Towards network-guided neuromodulation for epilepsy.</a> Brain. 145 (10), 3347-3362 (2022).</li><li>Bertram, E. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuronal+circuits+in+epilepsy:+Do+they+matter.">Neuronal circuits in epilepsy: Do they matter.</a> Exp Neurol. 244, 67-74 (2013).</li><li>Goodman, A. M., Szaflarski, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+advances+in+neuroimaging+of+epilepsy.">Recent advances in neuroimaging of epilepsy.</a> Neurotherapeutics. 18 (2), 811-826 (2021).</li><li>Caciagli, L., Bernhardt, B. C., Hong, S. J., Bernasconi, A., Bernasconi, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+network+alterations+and+their+structural+substrate+in+drug-resistant+epilepsy.">Functional network alterations and their structural substrate in drug-resistant epilepsy.</a> Front Neurosci. 8, 411 (2014).</li><li>Dai, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+microelectrode+arrays+for+detecting+multi-region+epileptic+activities+in+the+hippocampus+in+the+latent+period+of+rat+model+of+temporal+lobe+epilepsy.">In vivo microelectrode arrays for detecting multi-region epileptic activities in the hippocampus in the latent period of rat model of temporal lobe epilepsy.</a> Micromachines (Basel). 12 (6), 659 (2021).</li><li>Staba, R. J., Stead, M., Worrell, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiological+biomarkers+of+epilepsy.">Electrophysiological biomarkers of epilepsy.</a> Neurotherapeutics. 11 (2), 334-346 (2014).</li><li>Grienberger, C., Giovannucci, A., Zeiger, W., Portera-Cailliau, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-photon+calcium+imaging+of+neuronal+activity.">Two-photon calcium imaging of neuronal activity.</a> Nat Rev Methods Primers. 2 (1), 67 (2022).</li><li>Akerboom, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+a+gcamp+calcium+indicator+for+neural+activity+imaging.">Optimization of a gcamp calcium indicator for neural activity imaging.</a> J Neurosci. 32 (40), 13819-13840 (2012).</li><li>Zhang, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+and+sensitive+gcamp+calcium+indicators+for+imaging+neural+populations.">Fast and sensitive gcamp calcium indicators for imaging neural populations.</a> Nature. 615 (7954), 884-891 (2023).</li><li>Wu, Z., Lin, D., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pushing+the+frontiers:+Tools+for+monitoring+neurotransmitters+and+neuromodulators.">Pushing the frontiers: Tools for monitoring neurotransmitters and neuromodulators.</a> Nat Rev Neurosci. 23 (5), 257-274 (2022).</li><li>Vierock, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bipoles+is+an+optogenetic+tool+developed+for+bidirectional+dual-color+control+of+neurons.">Bipoles is an optogenetic tool developed for bidirectional dual-color control of neurons.</a> Nat Commun. 12 (1), 4527 (2021).</li><li>Luo, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acquiring+new+memories+in+neocortex+of+hippocampal-lesioned+mice.">Acquiring new memories in neocortex of hippocampal-lesioned mice.</a> Nat Commun. 13 (1), 1601 (2022).</li><li>Armstrong, C., Krook-Magnuson, E., Oijala, M., Soltesz, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Closed-loop+optogenetic+intervention+in+mice.">Closed-loop optogenetic intervention in mice.</a> Nat Protoc. 8 (8), 1475-1493 (2013).</li><li>Kraeuter, A. K., Guest, P. C., Sarnyai, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+open+field+test+for+measuring+locomotor+activity+and+anxiety-like+behavior.">The open field test for measuring locomotor activity and anxiety-like behavior.</a> Methods Mol Biol. 1916, 99-103 (2019).</li><li>Klapoetke, N. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Independent+optical+excitation+of+distinct+neural+populations.">Independent optical excitation of distinct neural populations.</a> Nat Methods. 11 (3), 338-346 (2014).</li><li>Vismer, M. S., Forcelli, P. A., Skopin, M. D., Gale, K., Koubeissi, M. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+piriform,+perirhinal,+and+entorhinal+cortex+in+seizure+generation.">The piriform, perirhinal, and entorhinal cortex in seizure generation.</a> Front Neural Circuits. 9, 27 (2015).</li><li>Chen, T. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasensitive+fluorescent+proteins+for+imaging+neuronal+activity.">Ultrasensitive fluorescent proteins for imaging neuronal activity.</a> Nature. 499 (7458), 295-300 (2013).</li><li>Birling, M. C., Dierich, A., Jacquot, S., Herault, Y., Pavlovic, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Highly-efficient,+fluorescent,+locus+directed+cre+and+flpo+deleter+mice+on+a+pure+c57bl/6n+genetic+background.">Highly-efficient, fluorescent, locus directed cre and flpo deleter mice on a pure c57bl/6n genetic background.</a> Genesis. 50 (6), 482-489 (2012).</li><li>Huang, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identifying+the+pathways+required+for+coping+behaviours+associated+with+sustained+pain.">Identifying the pathways required for coping behaviours associated with sustained pain.</a> Nature. 565 (7737), 86-90 (2019).</li><li>Likhtik, E., Stujenske, J. M., Topiwala, M. A., Harris, A. Z., Gordon, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prefrontal+entrainment+of+amygdala+activity+signals+safety+in+learned+fear+and+innate+anxiety.">Prefrontal entrainment of amygdala activity signals safety in learned fear and innate anxiety.</a> Nat Neurosci. 17 (1), 106-113 (2014).</li><li>Tavares, L. C. S., Tort, A. B. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hippocampal-prefrontal+interactions+during+spatial+decision-making.">Hippocampal-prefrontal interactions during spatial decision-making.</a> Hippocampus. 32 (1), 38-54 (2022).</li><li>Lu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optogenetic+dissection+of+ictal+propagation+in+the+hippocampal-entorhinal+cortex+structures.">Optogenetic dissection of ictal propagation in the hippocampal-entorhinal cortex structures.</a> Nat Commun. 7, 10962 (2016).</li><li>Liu, Q., Wu, Y., Wang, H., Jia, F., Xu, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Viral+tools+for+neural+circuit+tracing.">Viral tools for neural circuit tracing.</a> Neurosci Bull. 38 (12), 1508-1518 (2022).</li><li>Christenson Wick, Z., Krook-Magnuson, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Specificity,+versatility,+and+continual+development:+The+power+of+optogenetics+for+epilepsy+research.">Specificity, versatility, and continual development: The power of optogenetics for epilepsy research.</a> Front Cell Neurosci. 12, 151 (2018).</li><li>Paschen, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hippocampal+low-frequency+stimulation+prevents+seizure+generation+in+a+mouse+model+of+mesial+temporal+lobe+epilepsy.">Hippocampal low-frequency stimulation prevents seizure generation in a mouse model of mesial temporal lobe epilepsy.</a> Elife. 9, e54518 (2020).</li><li>Gummadavelli, A., Zaveri, H. P., Spencer, D. D., Gerrard, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expanding+brain-computer+interfaces+for+controlling+epilepsy+networks:+Novel+thalamic+responsive+neurostimulation+in+refractory+epilepsy.">Expanding brain-computer interfaces for controlling epilepsy networks: Novel thalamic responsive neurostimulation in refractory epilepsy.</a> Front Neurosci. 12, 474 (2018).</li><li>Vandekerckhove, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Technological+challenges+in+the+development+of+optogenetic+closed-loop+therapy+approaches+in+epilepsy+and+related+network+disorders+of+the+brain.">Technological challenges in the development of optogenetic closed-loop therapy approaches in epilepsy and related network disorders of the brain.</a> Micromachines (Basel). 12 (1), 38 (2020).</li><li>Li, Z. J., Zhang, L. B., Chen, Y. X., Hu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Advancements+and+challenges+in+neuromodulation+technology:+Interdisciplinary+opportunities+and+collaborative+endeavors.">Advancements and challenges in neuromodulation technology: Interdisciplinary opportunities and collaborative endeavors.</a> Sci Bull (Beijing). 68 (18), 1978-1982 (2023).</li><li>Zhang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+prototype+closed-loop+brain-machine+interface+for+the+study+and+treatment+of+pain.">A prototype closed-loop brain-machine interface for the study and treatment of pain.</a> Nat Biomed Eng. 7 (4), 533-545 (2023).</li><li>Varela, C., Wilson, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mpfc+spindle+cycles+organize+sparse+thalamic+activation+and+recently+active+ca1+cells+during+non-rem+sleep.">Mpfc spindle cycles organize sparse thalamic activation and recently active ca1 cells during non-rem sleep.</a> Elife. 9, e48881 (2020).</li><li>Adaikkan, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gamma+entrainment+binds+higher-order+brain+regions+and+offers+neuroprotection.">Gamma entrainment binds higher-order brain regions and offers neuroprotection.</a> Neuron. 102 (5), 929-943.e8 (2019).</li><li>Muthmann, J. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spike+detection+for+large+neural+populations+using+high+density+multielectrode+arrays.">Spike detection for large neural populations using high density multielectrode arrays.</a> Front Neuroinform. 9, 28 (2015).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>8-32 adapter</td>
			<td>Plexon</td>
			<td>Custom ordered</td>
			<td>Connect the female connector and headstage</td>
		</tr>
		<tr>
			<td>AAV-CaMKII&#945;-ChrimsonR-mcherry</td>
			<td>Taitool Bioscience</td>
			<td>S0371-9</td>
			<td>4 x 1012 VG/mL&#160;</td>
		</tr>
		<tr>
			<td>AAV-hsyn-Gcamp6m</td>
			<td>Taitool Bioscience</td>
			<td>S0471-9</td>
			<td>4 x 1012 VG/mL&#160;</td>
		</tr>
		<tr>
			<td>DAPI</td>
			<td>Sigma</td>
			<td>236276</td>
			<td>Titered 1:500</td>
		</tr>
		<tr>
			<td>Dental Cement</td>
			<td>New Century Dental</td>
			<td>430205</td>
			<td></td>
		</tr>
		<tr>
			<td>Electrophysiological recordings system</td>
			<td>Plexon</td>
			<td>Omniplex</td>
			<td></td>
		</tr>
		<tr>
			<td>Enameled wire</td>
			<td>N/A</td>
			<td>Custom ordered</td>
			<td>Diameter = 0.2 mm</td>
		</tr>
		<tr>
			<td>Female connector</td>
			<td>N/A</td>
			<td>Custom ordered</td>
			<td>1.25 mm pitch</td>
		</tr>
		<tr>
			<td>Glue</td>
			<td>Loctite</td>
			<td>45282</td>
			<td></td>
		</tr>
		<tr>
			<td>Laser</td>
			<td>Changchun New Industries</td>
			<td>BH81563</td>
			<td>635 nm&#160;</td>
		</tr>
		<tr>
			<td>MATLAB</td>
			<td>MathWorks</td>
			<td>R2021b</td>
			<td></td>
		</tr>
		<tr>
			<td>Microdrill</td>
			<td>RWD</td>
			<td>78001</td>
			<td></td>
		</tr>
		<tr>
			<td>Multichannel fiber photometry</td>
			<td>ThinkerTech</td>
			<td>FPS-SS-MC-LED</td>
			<td></td>
		</tr>
		<tr>
			<td>Optical fiber</td>
			<td>Xi&#39;an Bogao</td>
			<td>L-200UM</td>
			<td>Select the appropriate fiber length based on the depth of the targeted brain regions.</td>
		</tr>
		<tr>
			<td>PFA-Coated Tungsten wire</td>
			<td>A-M System</td>
			<td>795500</td>
			<td>Bare 0.002&#34;; Coated 0.0040&#34;</td>
		</tr>
		<tr>
			<td>Power meter</td>
			<td>Thorlabs</td>
			<td>PM100D</td>
			<td></td>
		</tr>
		<tr>
			<td>Stereotaxic Fxrame</td>
			<td>RWD</td>
			<td>68807</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue adhesive</td>
			<td>3M</td>
			<td>1469SB</td>
			<td></td>
		</tr>
	</tbody>
</table>

an integrated method for crafting flexible and convenient electrophysiological optrodes for multi-region in vivo recording

Epilepsy is a common neurological condition characterized by recurrent seizures, which manifest as convulsions, sensory disturbances, and loss of consciousness1. The pathophysiological mechanisms underlying epilepsy are complex and involve multiple interconnected brain regions2,3. Recent advances in neuroimaging have shed light on the large-scale networks involved in epilepsy4,5. However, understanding of the intricate circuitry and network mechanisms underlying the generation and propagation of epilepsy remains limited, partly due to the insufficient application of multi-region neural recording techniques6. Therefore, developing a flexible, integrated method capable of simultaneously monitoring neural activity across disparate brain regions is imperative.
Electrophysiological recordings are conducted to capture seizures and determine the presence of epilepsy7. Except for recording electrophysiological activity, there is a growing emphasis on the precise calcium activity of specific&#160;neural populations in epilepsy studies8,9. Advancements in calcium indicator synthesis and various probe designs have prompted researchers to adopt fiber photometry for capturing changes in neuronal activity and neural substances within the brain10,11. The two independent methods of detecting neuronal activity, namely electrophysiology and fiber photometry recording, complement each other, facilitating a more comprehensive understanding of dynamic neuronal processes.
Additionally, synchronous recording and modulating neural activity are pivotal for gaining insights into the brain functioning at both network and cellular levels. This approach enables researchers to observe and manipulate the brain&#39;s complex processes in real-time. Optogenetics has emerged as an indispensable tool for probing neural signaling, owing to its distinctive ability for selective stimulation or inhibition12. Despite widespread applications of multi-site electrophysiological recording in neuroscience13, the integration of multi-region electrophysiological recording with fiber photometry and optogenetic manipulation remains limited. More importantly, current methods for fabricating and implanting multi-region electrodes lack flexibility14. These limitations hinder our capacity to dissect specific circuit functions and interactions across multiple regions. Here, we present a cost-effective and convenient multi-region in vivo recording approach that sheds light on neuronal processes across regions in kindling-induced seizures and other neuropsychiatric disorders.

<meta charset="utf8"></head><body>Yazar Spotlight: Sağlık ve Hastalıkta Sinirsel İletişim ve Devre Etkileşimlerinin Çözülmesi

Çok Bölgeli İn Vivo Kayıt için Esnek ve Kullanışlı Elektrofizyolojik Seçenekler Hazırlamak için Entegre Bir Yöntem

Epilepsy is a neurological disorder characterized by synchronized abnormal discharges involving multiple brain regions. Focal lesions facilitate the ...

an-integrated-method-for-crafting-flexible-convenient

Research

JoVE Journal

Neuroscience

An Integrated Method for Crafting Flexible and Convenient Electrophysiological Optrodes for Multi-Region In Vivo Recording

432 Görüntüleme.  Fudan University. Çok kanallı elektrofizyolojik kayıt, fiber fotometri, nöro modülasyon ve fonksiyonel manyetik rezonans görüntüleme gibi teknikleri kapsayan multimodal yaklaşımlar kullanarak beyin ağlarını ve sinir devrelerini araştırıyoruz. Farklı beyin bölgelerinin hem sağlıklı hem de hastalıklı durumlarda in vivo olarak nasıl iletişim kurduğunu ve etkileşime girdiğini cevaplamaya çalışıyoruz. Moleküler hücresel döngüleri ve ağ katmanlar...

Video: Yazar Spotlight: Sağlık ve Hastalıkta Sinirsel İletişim ve Devre Etkileşimlerinin Çözülmesi

Epilepsy is a neurological disorder characterized by synchronized abnormal discharges involving multiple brain regions. Focal lesions facilitate the propagation of epileptic signals through associated neural circuits. Therefore, in vivo recording of local field potential (LFP) from the critical brain regions is&#160;essential for deciphering the circuits involved in seizure propagation. However, current methods for electrode fabrication and implantation lack flexibility. Here, we present a handy device designed for electrophysiological recordings (LFPs and electroencephalography [EEG]) across multiple regions. Additionally, we seamlessly integrated optogenetic manipulation and calcium signaling recording with LFP recording. Robust after-discharges were observed in several separate regions during epileptic seizures, accompanied by increasing&#160;calcium signaling. The approach used in this study offers a convenient and flexible strategy for synchronous neural recordings across diverse regions of the brain. It holds the potential for advancing research on neurological disorders by providing insights into the neural profiles of multiple regions involved in these disorders.

We have developed a simplified and cost-effective approach for electrode fabrication and conducted recordings of signals across multiple regions in freely moving mice. Utilizing optogenetics, alongside multi-region electrophysiology and calcium signal recording, enabled the revelation of neuronal activities across regions in the seizure kindling model.