JoVE Logo
Faculty Resource Center

Sign In

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

DOI :

10.3791/58365-v

October 30th, 2018

October 30th, 2018

8,818 Views

1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, 3Neuroscience Program, NYU School of Medicine

Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.

-- Views

Related Videos

article

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

article

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

article

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

article

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

article

Author Spotlight: Advancing Lens Biomechanics Research Through a Novel Protocol for Imaging Complex Interdigitations and Protein Staining

article

Author Spotlight: Effect of Left Atrial Ligation on Avian Embryonic Hearts and HLHS Implications

article

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

article

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

article

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

article

Author Spotlight: Advancing Research Through Single Cell Sequencing and Spatial Histology in Placental Tissues

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved