Neuronal Bouton Labeling with Fluorescently Labeled Primary Antibody Against HRP
5:23
Perisynaptic Space Labeling with Dextran Fluorophore Conjugate
6:12
Mounting the Tissue for Visualization Under a Confocal Microscope
7:23
Representative Results
10:17
Conclusion
We described structural features of the Glia-neuromuscular synapses in a novel Inside-out tissue preparation of live fly larvae using fluorescent dyes with confocal microscopy. We labeled live neuron terminals with fluorescent primary antibodies to HRP, and also visualized the perisynaptic space with fluorescent Dextrans.