JoVE Logo
Faculty Resource Center

Sign In

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

DOI :

10.3791/1170-v

April 13th, 2009

April 13th, 2009

8,022 Views

1Whitehead Institute for Biomedical Research, MIT - Massachusetts Institute of Technology

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 3 describes the process of fluorescently labeling the amplified RNA from both host and viral samples by amino allyl coupling of dyes. Part 3 of 3.

Tags

Vaccinia Virus Infection

-- Views

Related Videos

article

Bacterial Gene Expression Analysis Using Microarrays

article

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

article

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

article

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

article

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

article

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

article

In vitro Reconstitution of the Active T. castaneum Telomerase

article

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

article

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

article

The MultiBac Protein Complex Production Platform at the EMBL

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved