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Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

DOI :

10.3791/52428-v

February 16th, 2015

February 16th, 2015

9,603 Views

1Department of Biology, Ball State University, 2Division of Nephrology, Cincinnati Children's Hospital

This article describes a yeast growth-based assay for the determination of genetic requirements for protein degradation. It also demonstrates a method for rapid extraction of yeast proteins, suitable for western blotting to biochemically confirm degradation requirements. These techniques can be adapted to monitor degradation of a variety of proteins.

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Keywords Protein Degradation

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