September 17th, 2009
•Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.
Tags
Related Videos
Primary Culture of Adult Rat Heart Myocytes
Dissection of Hippocampal Dentate Gyrus from Adult Mouse
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
DNA Electroporation, Isolation and Imaging of Myofibers
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved