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20.1K Views
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11:44 min
June 16th, 2009
DOI :
10.3791/1308-v
Chapters
0:04
Title
0:52
Introduction
1:18
Preparation for Surgery
4:06
The Rat Heart Dissection
5:05
Myocytes Isolation
10:15
Myocyte Cell Culture
10:56
Representative Outcome: Cultured Myocytes
11:20
Conclusion
Summary
In this paper, we described a typical way to isolate and culture adult rat heart myocytes. Collagenase and protease are used to digest and isolate single myocytes. Myocytes cultured follow this protocol meet most experiment requirements.
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