January 4th, 2010
•Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.
Tags
Related Videos
Preparation of Drosophila S2 cells for Light Microscopy
Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved