JoVE Logo
Faculty Resource Center

Sign In

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

DOI :

10.3791/61434-v

October 31st, 2020

October 31st, 2020

5,574 Views

1Department of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute, Istituto di Ricerche Farmacologiche Mario Negri IRCCS

This protocol shows how to employ super-resolution microscopy to study protein co-localization in primary neuronal cultures.

Tags

Super resolution Imaging

-- Views

Related Videos

article

Laser Capture Microdissection of Drosophila Peripheral Neurons

article

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

article

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

article

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

article

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

article

Super-resolution Imaging of Neuronal Dense-core Vesicles

article

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

article

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

article

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

article

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved