JoVE Logo
Faculty Resource Center

Sign In

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

DOI :

10.3791/1852-v

March 22nd, 2010

March 22nd, 2010

28,095 Views

1Department of Chemical Engineering and Materials Science, City of Hope Cancer Center

Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

article

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

article

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

article

Induction and Analysis of Epithelial to Mesenchymal Transition

article

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

article

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

article

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

article

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

article

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved