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Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs Video (Video) | JoVE

DOI :

10.3791/1852-v

March 22nd, 2010

March 22nd, 2010

28,041 Views

1Department of Chemical Engineering and Materials Science, City of Hope Cancer Center

Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.

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